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Author Li K., Cheng Y., Fang C.
Title <i>OsDWARF10</i>, transcriptionally repressed by <i>OsSPL3</i>, regulates the nutritional metabolism of polished rice.
Abstract:
Strigolactone (SL) plays essential roles in plant development and the metabolism of rice leaves. However, the impact of SL on the accumulation of nutritional metabolites in polished rice, as well as the transcription factors directly involved in SL synthesis, remains elusive. In this study, we performed a metabolome analysis on polished rice samples from mutants of an SL biosynthetic gene, <i>OsDWARF10</i> (<i>OsD10</i>). Compared with those in the wild type plants, primary and secondary metabolites exhibited a series of alterations in the <i>d10</i> mutants. Notably, the <i>d10</i> mutants showed a substantial increase in the amino acids and vitamins content. Through a yeast one-hybridization screening assay, we identified OsSPL3 as a transcription factor that binds to the <i>OsD10</i> promoter, thereby inhibiting <i>OsD10</i> transcription <i>in vivo</i> and <i>in vitro</i>. Furthermore, we conducted a metabolic profiling analysis in polished rice from plants that overexpressed <i>OsSPL3</i> and observed enhanced levels of amino acids and vitamins. This study identified a novel transcriptional repressor of the SL biosynthetic gene and elucidated the regulatory roles of OsSPL3 and <i>OsD10</i> on the accumulation of nutritional metabolites in polished rice.
Journal Front Plant Sci
Country China
Volume 14
Pages 1322463
Year 2023
PubMed ID 38130489
PubMed Central ID 10733476
DOI 10.3389/fpls.2023.1322463
URL -
Relation
Gene D10 LCRN1
INSD -
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain 		-
Stages in Each Organ
- Muant Lines (Gene) 		-
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
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