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Reference Detail
| Reference | ||
|---|---|---|
| Author | Zheng X., Yuan Z., Yu Y., Yu S., He H. | |
| Title | <i>OsCSD2</i> and <i>OsCSD3</i> Enhance Seed Storability by Modulating Antioxidant Enzymes and Abscisic Acid in Rice. | |
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Abstract: Seed deterioration during storage poses a significant challenge to rice production, leading to a drastic decline in both edible quality and viability, thereby impacting overall crop yield. This study aimed to address this issue by further investigating candidate genes associated with two previously identified QTLs for seed storability through genome association analysis. Among the screened genes, two <i>superoxide dismutase</i> (<i>SOD</i>) genes, <i>OsCSD2</i> (<i>Copper/zinc superoxide dismutase 2</i>) and <i>OsCSD3</i>, were selected for further study. The generation of overexpression and CRISPR/Cas9 mutant transgenic lines revealed that <i>OsCSD2</i> and <i>OsCSD3</i> play a positive regulatory role in enhancing rice seed storability. Subsequent exploration of the physiological mechanisms demonstrated that overexpression lines exhibited lower relative electrical conductivity, indicative of reduced cell membrane damage, while knockout lines displayed the opposite trend. Furthermore, the overexpression lines of <i>OsCSD2</i> and <i>OsCSD3</i> showed significant increases not only in SOD but also in CAT and POD activities, highlighting an augmented antioxidant system in the transgenic seeds. Additionally, hormone profiling indicated that ABA contributed to the improved seed storability observed in these lines. In summary, these findings provide valuable insights into the regulatory mechanisms of <i>OsCSDs</i> in rice storability, with potential applications for mitigating grain loss and enhancing global food security. |
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| Journal | Plants (Basel) | |
| Country | China | |
| Volume | 13(2) | |
| Pages | ||
| Year | 2024 | |
| PubMed ID | 38276765 | |
| PubMed Central ID | 10818270 | |
| DOI | 10.3390/plants13020310 | |
| URL | - | |
| Relation | ||
| Gene | SOD2 SODCC2 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
