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Reference Detail
| Reference | ||
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| Author | Lin S., Liu Z., Sun S., Xue F., Li H., Tursun A., Cao L., Zhang L., Zoe A Wilson, Zhang D., Liang W. | |
| Title | Rice HEAT SHOCK PROTEIN60-3B maintains male fertility under high temperature by starch granule biogenesis. | |
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Abstract: Heat stress has a deleterious effect on male fertility in rice (Oryza sativa), but mechanisms to protect against heat stress in rice male gametophytes are poorly understood. Here, we have isolated and characterized a heat-sensitive male-sterile rice mutant, heat SHOCK PROTEIN60-3B (oshsp60-3b), that shows normal fertility at optimal temperatures but decreasing fertility as temperatures increase. High temperatures interfered with pollen starch granule formation and reactive oxygen species (ROS) scavenging in oshsp60-3b anthers, leading to cell death and pollen abortion. In line with the mutant phenotypes, oshsp60-3b was rapidly upregulated in response to heat shock and its protein products were localized to the plastid. Critically, overexpression of oshsp60-3b enhanced the heat tolerance of pollen in transgenic plants. We demonstrated that oshsp60-3b interacted with FLOURY ENDOSPERM6(FLO6) in plastids, a key component involved in the starch granule formation in the rice pollen. Western blot results showed that FLO6 level was substantially decreased in oshsp60-3b anthers at high temperature, indicating that oshsp60-3b is required to stabilize FLO6 when temperatures exceed optimal conditions. We suggest that in response to high temperature, oshsp60-3b interacts with FLO6 to regulate starch granule biogenesis in rice pollen and attenuates ROS levels in anthers to ensure normal male gametophyte development in rice. |
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| Journal | Plant Physiol. | |
| Country | China | |
| Volume | 192(3) | |
| Pages | 2301-2317 | |
| Year | 2023 | |
| PubMed ID | 36861636 | |
| PubMed Central ID | 10315285 | |
| DOI | 10.1093/plphys/kiad136 | |
| URL | - | |
| Relation | ||
| Gene | FLO6 HSP60-3B | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
