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Reference Detail
| Reference | ||
|---|---|---|
| Author | Jia Q., Zhang X., Liu Q., Li J., Wang W., Ma X., Zhu B., Li S., Gong S., Tian J., Yuan M., Zhao Y., Zhou D.X. | |
| Title | A DNA adenine demethylase impairs PRC2-mediated repression of genes marked by a specific chromatin signature. | |
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Abstract: The Fe (II)- and α-ketoglutarate-dependent AlkB family dioxygenases are implicated in nucleotide demethylation. AlkB homolog1 (ALKBH1) is shown to demethylate DNA adenine methylation (6mA) preferentially from single-stranded or unpaired DNA, while its demethylase activity and function in the chromatin context are unclear. Here, we find that loss-of-function of the rice ALKBH1 gene leads to increased 6mA in the R-loop regions of the genome but has a limited effect on the overall 6mA level. However, in the context of mixed tissues, rather than on individual loci, the ALKBH1 mutation or overexpression mainly affects the expression of genes with a specific combination of chromatin modifications in the body region marked with H3K4me3 and H3K27me3 but depleted of DNA CG methylation. In the similar context of mixed tissues, further analysis reveals that the ALKBH1 protein preferentially binds to genes marked by the chromatin signature and has a function to maintain a high H3K4me3/H3K27me3 ratio by impairing the binding of Polycomb repressive complex 2 (PRC2) to the targets, which is required for both the basal and stress-induced expression of the genes. Our findings unravel a function of ALKBH1 to control the balance between the antagonistic histone methylations for gene activity and provide insight into the regulatory mechanism of PRC2-mediated H3K27me3 deposition within the gene body region. |
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| Journal | Genome Biol. | |
| Country | China | |
| Volume | 24(1) | |
| Pages | 198 | |
| Year | 2023 | |
| PubMed ID | 37649077 | |
| PubMed Central ID | 10469495 | |
| DOI | 10.1186/s13059-023-03042-4 | |
| URL | - | |
| Relation | ||
| Gene | 2R_MYB36 ALKBH1 ALKBH10 ALKBH1A ALKBH2 ALKBH5 ALKBH8A ALKBH8B CDPK4 CYP94C2B EMF2B NAC3 WRKY53 WRKY71 ZFP182 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
