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Author Okumura T., Kitajima T., Kaji T., Urano H., Matsumoto K., Inagaki H., Miyamoto K., Okada K., Ueda M.
Title Difference in the ligand affinity among redundant plant hormone receptors of rice OsCOI1a/1b/2-OsJAZs.
Abstract:
(3R, 7S)-jasmonoyl-L-isoleucine (JA-Ile) is a lipid-derived plant hormone that regulates plant responses, including biotic/abiotic stress adaptation. In the plant cells, JA-Ile is perceived by COI1-JAZ co-receptor by causing protein-protein interaction between COI1 and JAZ proteins to trigger gene expressions. In this study, we focused on Oryza sativa, a model monocot and an important crop, with 45 possible OsCOI-OsJAZ co-receptor pairs composed of three OsCOI homologs (OsCOI1a, OsCOI1b, and OsCOI2) and 15 OsJAZ homologs. We performed fluorescein anisotropy and pull-down assays to examine the affinity between JA-Ile and OsCOI1a/1b/2-OsJAZ1-15 co-receptor pairs. The results revealed a remarkable difference in the modes of ligand perception by OsCOI1a/1b and OsCOI2. Recently, the unique function of OsCOI2 in some of the JA-responses were revealed. Our current results will lead to the possible development of OsCOI2-selective synthetic ligand.
Journal Biosci. Biotechnol. Biochem.
Country Japan
Volume 87(10)
Pages 1122-1128
Year 2023
PubMed ID 37403366
PubMed Central ID -
DOI 10.1093/bbb/zbad092
URL -
Relation
Gene COI1 COI1B COI2 EG2 TIFY10A TIFY10B TIFY10C TIFY11A TIFY11B TIFY11C TIFY11D TIFY11E TIFY11F TIFY11G TIFY6A TIFY6B TIFY8 TIFY9
INSD -
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
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Stages in Each Organ
- Muant Lines (Gene)
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Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase