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| Reference | ||
|---|---|---|
| Author | Liu Y., Zhang H., Feng W., Lin X., Gao A., Cao Y., Yang Q., Wang Y., Li W., Fu F., Yu H. | |
| Title | The Maize ZmBES1/BZR1-9 Transcription Factor Accelerates Flowering in Transgenic <i>Arabidopsis</i> and Rice. | |
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Abstract: In model plants, the BRI1-EMS suppressor 1 (BES1)/brassinazole-resistant 1 (BZR1) transcription factors play vital roles in regulating growth, development, and stimuli response. However, the roles of maize <i>ZmBES1/BZR1</i> members are largely unknown. In this research, the <i>ZmBES1/BZR1-9</i> gene was ectopically expressed in <i>Arabidopsis</i> and rice for the phenotyping of flowering. We found that the complementation and overexpression of <i>ZmBES1/BZR1-9</i> in <i>BES1-D</i> mutant and wild type <i>Arabidopsis</i> both resulted in early flowering that was about 10 days shorter than in the untransformed control under long-day conditions. In addition, there was no difference in the rosette leaf number between all transgenic lines and the control. Subsequently, the <i>ZmBES1/BZR1-9</i> gene was overexpressed in rice. It was found that overexpression lines of rice exhibited early flowering with heading dates that were 8 days shorter compared with untransformed plants. Moreover, the results of RNA-seq and qRT-PCR showed that five flowering-regulated genes, namely <i>At2-MMP</i>, <i>AtPCC1</i>, <i>AtMYB56</i>, <i>AtPELPK1</i>, and <i>AtPRP10,</i> were significantly up-regulated in all complementary and overexpressing lines of <i>Arabidopsis</i>. Meanwhile, the results of RNA-seq showed that 69 and 33 differentially expressed genes (DEGs) were up- and down-regulated in transgenic rice, respectively. Four flowering-related genes, namely <i>OsGA20OX1</i>, <i>OsCCR19</i>, <i>OsBTBN19</i>, and <i>OsRNS4</i> were significantly up-regulated in transgenic lines. To sum up, our findings demonstrate that <i>ZmBES1/BZR1-9</i> is involved in controlling flowering and provide insights into further underlying roles of <i>BES1/BZR1s</i> in regulating growth and development in crops. |
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| Journal | Plants (Basel) | |
| Country | China | |
| Volume | 12(16) | |
| Pages | ||
| Year | 2023 | |
| PubMed ID | 37631206 | |
| PubMed Central ID | 10459471 | |
| DOI | 10.3390/plants12162995 | |
| URL | - | |
| Relation | ||
| Gene | BTBN19 CCR19 GNP1 KCS2 RNS4 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
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Stages in Each Organ - Muant Lines (Gene) |
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| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
