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Reference Detail
| Reference | ||
|---|---|---|
| Author | Tang X., Zhong W., Wang K., Gong X., Xia Y., Nong J., Xiao L., Xia S. | |
| Title | Regulation of Grain Chalkiness and Starch Metabolism by FLO2 interaction factor 3, a bHLH transcription factor in <i>Oryza sativa</i>. | |
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Abstract: Chalkiness is a key determinant that directly affects the appearance and cooking quality of rice grains. Previously, <i>Floury endosperm 2</i> (<i>FLO2</i>) was reported to be involved in the formation of rice chalkiness; however, its regulation mechanism is still unclear. Here, FLO2 interaction factor 3 (<i>Os</i>FIF3), a bHLH transcription factor, was identified and analyzed in <i>Oryza sativa</i>. A significant increase in chalkiness was observed in <i>OsFIF3</i>-overexpressed grains, coupled with a round, hollow filling of starch granules and reduced grain weight. <i>Os</i>FIF3 is evolutionarily conserved in monocotyledons, but variable in dicotyledons. Subcellular localization revealed the predominant localization of <i>Os</i>FIF3 in the nucleus. The DAP-seq (DNA affinity purification sequencing) results showed that <i>Os</i>FIF3 could affect the transcriptional accumulation of <i>β-amylase 1</i>, <i>α-amylase isozyme 2A-like</i>, <i>pectinesterase 11</i>, <i>β-glucosidase 28 like</i>, <i>pectinesterase</i>, <i>sucrose transport protein 1</i> (<i>SUT1</i>), and <i>FLO2</i> through the binding of the CACGTG motif on their promoters. Moreover, <i>FLO2</i> and <i>SUT1</i> with abundant <i>Os</i>FIF3 binding signals showed significant expression reduction in <i>OsFIF3</i> overexpression lines, further confirming <i>Os</i>FIF3's role in starch metabolism regulation and energy material allocation. Taken together, these findings show that the overexpression of <i>OsFIF3</i> inhibits the expression of <i>FLO2</i> and <i>SUT1</i>, thereby increasing grain chalkiness and affecting grain weight. |
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| Journal | Int J Mol Sci | |
| Country | China | |
| Volume | 24(16) | |
| Pages | ||
| Year | 2023 | |
| PubMed ID | 37628959 | |
| PubMed Central ID | 10454616 | |
| DOI | 10.3390/ijms241612778 | |
| URL | - | |
| Relation | ||
| Gene | AMY2A BAMY1 BGLU28 BHLH182 DPE1 FLO2 PME4 SUT1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
