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Reference Detail
| Reference | ||
|---|---|---|
| Author | Shi Y., Jiang N., Wang M., Du Z., Chen J., Huang Y., Li M., Jin Y., Li J., Wan J., Jin X., Zhang L., Huang J. | |
| Title | <i>OsHIPP17</i> is involved in regulating the tolerance of rice to copper stress. | |
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Abstract: Heavy metal-associated isoprenylated plant proteins (HIPPs) play vital roles in metal absorption, transport and accumulation in plants. However, so far, only several plant HIPPs have been functionally analyzed. In this study, a novel HIPP member <i>OsHIPP17</i>, which was involved in the tolerance to copper (Cu) was functionally characterized. In this study, qRT-PCR, Yeast transgenic technology, Plant transgenic technology, ICP-MS and so on were used for research. OsHIPP17 protein was targeted to the nucleus. The Cu concentration reached 0.45 mg/g dry weight due to the overexpression of <i>OsHIPP17</i> in yeast cells. Meanwhile, the overexpression of <i>OsHIPP17</i> resulted in the compromised growth of <i>Arabidopsis thaliana</i> (<i>Arabidopsis</i>) under Cu stress. The root length of <i>OsHIPP17</i> mutant lines was also significantly reduced by 16.74- 24.36% under 25 mM Cu stress. The roots of <i>OsHIPP17</i> rice mutant showed increased Cu concentration by 7.25%-23.32%. Meanwhile, knockout of OsHIPP17 decreased the expression levels of <i>OsATX1</i>, <i>OsZIP1</i>, <i>OsCOPT5</i> or <i>OsHMA5</i>, and increased the expression levels of <i>OsCOPT1</i> or <i>OsHMA4</i>. Antioxidant enzyme activity was also reduced in rice due to the knockout of <i>OsHIPP17</i>. Moreover, the expression levels of cytokinin-related genes in plants under Cu stress were also affected by overexpression or knockout of <i>OsHIPP17</i>. These results implied that <i>OsHIPP17</i> might play a role in plant Cu toxic response by affecting the expression of Cu transport genes or cytokinin-related genes. Simultaneously, our work may shed light on the underlying mechanism of how heavy metals affect the plant growth and provide a novel rice genetic source for phytoremediation of heavy metal-contaminated soil. |
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| Journal | Front Plant Sci | |
| Country | China | |
| Volume | 14 | |
| Pages | 1183445 | |
| Year | 2023 | |
| PubMed ID | 37484470 | |
| PubMed Central ID | 10359898 | |
| DOI | 10.3389/fpls.2023.1183445 | |
| URL | - | |
| Relation | ||
| Gene | ATX1 CKX1 COPT1 COPT5 HIPP17 HMA4 HMA5 IPT1 RR1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
