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Reference Detail
| Reference | ||
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| Author | Chen T., Xiao W., Huang C., Zhou D., Liu Y., Guo T., Chen Z., Wang H. | |
| Title | Fine Mapping of the Affecting Tillering and Plant Height Gene <i>CHA-1</i> in Rice. | |
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Abstract: The plant architecture of rice is an important factor affecting yield. Strigolactones (SLs) are newly discovered carotenoid-derived plant hormones that play an important role in rice plant architecture. In this study, a high-tillering dwarf mutant, <i>CHA-1,</i> was identified by spatial mutagenesis. <i>CHA-1</i> was located in the region of 31.52-31.55 MB on chromosome 1 by map-based cloning. Compared with the wild-type THZ, the <i>CHA-1</i> mutant showed that ACCAC replaced TGGT in the coding region of the candidate gene <i>LOC_Os01g54810</i>, leading to premature termination of expression. Genetic complementation experiments proved that <i>LOC_Os01g54810</i> was <i>CHA-1</i>, which encodes a putative member of Class III lipase. Expression analysis showed that <i>CHA-1</i> was constitutively expressed in various organs of rice. Compared with those in THZ, the expression levels of the <i>D17</i> and <i>D10</i> genes were significantly downregulated in the <i>CHA-1 mutant</i>. In addition, the concentrations of ent-2'-epi-5-deoxystrigol (epi-5DS) in the root exudates of the <i>CHA-1</i> mutant was significantly reduced compared with that of THZ, and exogenous application of GR24 inhibited the tillering of the <i>CHA-1</i> mutant. These results suggest that <i>CHA-1</i> influences rice architecture by affecting SL biosynthesis. |
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| Journal | Plants (Basel) | |
| Country | China | |
| Volume | 12(7) | |
| Pages | ||
| Year | 2023 | |
| PubMed ID | 37050133 | |
| PubMed Central ID | 10096525 | |
| DOI | 10.3390/plants12071507 | |
| URL | - | |
| Relation | ||
| Gene | D10 D14 D17 D3 THIS1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
