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Reference Detail
| Reference | ||
|---|---|---|
| Author | Zhao L., Oyagbenro R., Feng Y., Xu M., Peters R.J. | |
| Title | Oryzalexin S biosynthesis: a cross-stitched disappearing pathway. | |
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Abstract: Rice produces many diterpenoid phytoalexins and, reflecting the importance of these natural products in this important cereal crop plant, its genome contains three biosynthetic gene clusters (<i>BGCs</i>) for such metabolism. The chromosome 4 <i>BGC</i> (<i>c4BGC</i>) is largely associated with momilactone production, in part due to the presence of the initiating <i>syn</i>-copalyl diphosphate (CPP) synthase gene (<i>OsCPS4</i>). Oryzalexin S is also derived from <i>syn</i>-CPP. However, the relevant subsequently acting <i>syn</i>-stemarene synthase gene (<i>OsKSL8</i>) is not located in the <i>c4BGC</i>. Production of Oryzalexin S further requires hydroxylation at carbons 2 and 19 (C2 and C19), presumably catalyzed by cytochrome P450 (CYP) monooxygenases. Here it is reported the closely related CYP99A2 and CYP99A3, whose genes are also found in the <i>c4BGC</i> catalyze the necessary C19-hydroxylation, while the closely related CYP71Z21 and CYP71Z22, whose genes are found in the recently reported chromosome 7 <i>BGC</i> (<i>c7BGC</i>), catalyze subsequent hydroxylation at C2α. Thus, Oryzalexin S biosynthesis utilizes two distinct <i>BGCs</i>, in a pathway cross-stitched together by <i>OsKSL8</i>. Notably, in contrast to the widely conserved <i>c4BGC</i>, the <i>c7BGC</i> is subspecies (ssp.) specific, being prevalent in ssp. japonica and only rarely found in the other major ssp. indica. Moreover, while the closely related <i>syn</i>-stemodene synthase <i>OsKSL11</i> was originally considered to be distinct from <i>OsKSL8</i>, it has now been reported to be a ssp. indica derived allele at the same genetic loci. Intriguingly, more detailed analysis indicates that <i>OsKSL8(j)</i> is being replaced by <i>OsKSL11</i> (<i>OsKSL8i</i>), suggesting introgression from ssp. indica to (sub)tropical japonica, with concurrent disappearance of Oryzalexin S production. The online version contains supplementary material available at 10.1007/s42994-022-00092-3. |
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| Journal | Abiotech | |
| Country | unknown | |
| Volume | 4(1) | |
| Pages | 1-7 | |
| Year | 2023 | |
| PubMed ID | 37220540 | |
| PubMed Central ID | 10199973 | |
| DOI | 10.1007/s42994-022-00092-3 | |
| URL | - | |
| Relation | ||
| Gene | CYP71Z21 CYP71Z22 CYP99A2 CYP99A3 KS8 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
