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Reference Detail
| Reference | ||
|---|---|---|
| Author | Kim M.S., Ko S.R., Jung Y.J., Kang K.K., Lee Y.J., Cho Y.G. | |
| Title | Knockout Mutants of <i>OsPUB7</i> Generated Using CRISPR/Cas9 Revealed Abiotic Stress Tolerance in Rice. | |
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Abstract: Plants produce and accumulate stress-resistant substances when exposed to abiotic stress, which involves a protein conversion mechanism that breaks down stress-damaged proteins and supplies usable amino acids. Eukaryotic protein turnover is mostly driven by the ubiquitination pathway. Among the three enzymes required for protein degradation, E3 ubiquitin ligase plays a pivotal role in most cells, as it determines the specificity of ubiquitination and selects target proteins for degradation. In this study, to investigate the function of <i>OsPUB7</i> (Plant U-box gene in <i>Oryza sativa</i>), we constructed a CRISPR/Cas9 vector, generated <i>OsPUB7</i> gene-edited individuals, and evaluated resistance to abiotic stress using gene-edited lines. A stress-tolerant phenotype was observed as a result of drought and salinity stress treatment in the T2<i>OsPUB7</i> gene-edited null lines (PUB7-GE) lacking the T-DNA. In addition, although PUB7-GE did not show any significant change in mRNA expression analysis, it showed lower ion leakage and higher proline content than the wild type (WT). Protein-protein interaction analysis revealed that the expression of the genes (<i>OsPUB23</i>, <i>OsPUB24</i>, <i>OsPUB66</i>, and <i>OsPUB67</i>) known to be involved in stress increased in PUB7-GE and this, by forming a 1-node network with <i>OsPUB66</i> and <i>OsPUB7</i>, acted as a negative regulator of drought and salinity stress. This result provides evidence that <i>OsPUB7</i> will be a useful target for both breeding and future research on drought tolerance/abiotic stress in rice. |
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| Journal | Int J Mol Sci | |
| Country | South Korea | |
| Volume | 24(6) | |
| Pages | ||
| Year | 2023 | |
| PubMed ID | 36982409 | |
| PubMed Central ID | 10048836 | |
| DOI | 10.3390/ijms24065338 | |
| URL | - | |
| Relation | ||
| Gene | PUB23 PUB24 PUB44 PUB58 PUB66 PUB67 PUB7 PUB73 RLCK93 RSSB4 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
