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Reference Detail
| Reference | ||
|---|---|---|
| Author | Zhang Y., Gan L., Zhang Y., Huang B., Wan B., Li J., Tong L., Zhou X., Wei Z., Li Y., Song Z., Zhang X., Cai D., He Y. | |
| Title | OsCBL5-CIPK1-pp23 module enhances rice grain size and weight through the gibberellin pathway. | |
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Abstract: grain size is a key factor in determining rice (Oryza sativa) yield, and exploring new pathways to regulate grain size has immense potential to improve yield. In this study, we report that OsCBL5 encodes a calcineurin B subunit protein that significantly promotes grain size and weight. OsCBL5 plants produced obviously smaller and lighter seeds. We further revealed that OsCBL5 promotes grain size by affecting cell expansion in the spikelet hull. Biochemical analyses demonstrated that CBL5 interacts with CIPK1 and pp23. Furthermore, double and triple mutations were induced using CRISPR/Cas9 (cr) to analyze the genetic relationship. It was found that the cr-CBL5/CIPK1 phenotype was similar to that of cr-CIPK1 and that the cr-CBL5/pp23, cr-CIPK1/pp23, and cr-CBL5/CIPK1/pp23 phenotype was similar to that of cr-pp23, indicating that OsCBL5, CIPK1, and pp23 act as a molecular module influencing seed size. In addition, the results show that both CBL5 and CIPK1 are involved in the gibberellic acid (GA) pathway and significantly affect the accumulation of endogenous active GA4 . pp23 participates in GA signal transduction. In brief, this study identified a new module that affects rice grain size, OsCBL5-CIPK1-pp23, which could potentially be targeted to improve rice yield. |
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| Journal | Plant J. | |
| Country | China | |
| Volume | ||
| Pages | ||
| Year | 2023 | |
| PubMed ID | 37133258 | |
| PubMed Central ID | - | |
| DOI | 10.1111/tpj.16266 | |
| URL | - | |
| Relation | ||
| Gene | CBL5 CIPK01 CIPK03 CIPK04 CIPK18 CPS1 D18 D35 EXPA10 EXPA4 EXPB2 EXPB5 GA20OX3 GA2OX1 GID1 GS2 GS5 GW6 GW7 ILI6 KAO KS1 PP23 SLR1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
