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Reference Detail
| Reference | ||
|---|---|---|
| Author | Huang P., Ding Z., Duan M., Xiong Y., Li X., Yuan X., Huang J. | |
| Title | <i>OsLUX</i> Confers Rice cold tolerance as a Positive Regulatory Factor. | |
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Abstract: During the early seedling stage, rice (<i>Oryza sativa</i> L.) must overcome low-temperature stress. While a few cold-tolerance genes have been characterized, further excavation of cold-resistance genes is still needed. In this study, we identified a cold-induced transcription factor-LUX ARRHYTHMO (LUX)-in rice. OsLUX was found to be specifically expressed in leaf blades and upregulated by both cold stress and circadian rhythm. The full-length OsLUX showed autoactivation activity, and the OsLUX protein localized throughout the entire onion cell. Overexpressing <i>OsLUX</i> resulted in increased cold tolerance and reduced ion leakage under cold-stress conditions during the seedling stage. In contrast, the knockout of <i>OsLUX</i> decreased seedling cold tolerance and showed higher ion leakage compared to the wild type. Furthermore, overexpressing <i>OsLUX</i> upregulated the expression levels of oxidative stress-responsive genes, which improved reactive oxygen species (ROS) scavenging ability and enhanced tolerance to chilling stress. Promoter analysis showed that the <i>OsLUX</i> promoter contains two dehydration-responsive element binding (DREB) motifs at positions -510/-505 (GTCGGa) and -162/-170 (cCACCGccc), which indicated that OsDREB1s and OsDREB2s probably regulate <i>OsLUX</i> expression by binding to the motif to respond to cold stress. Thus, OsLUX may act as a downstream gene of the DREB pathway. These results demonstrate that OsLUX serves as a positive regulatory factor of cold stress and that overexpressing OsLUX could be used in rice breeding programs to enhance abiotic stress tolerance. |
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| Journal | Int J Mol Sci | |
| Country | China | |
| Volume | 24(7) | |
| Pages | ||
| Year | 2023 | |
| PubMed ID | 37047700 | |
| PubMed Central ID | 10094877 | |
| DOI | 10.3390/ijms24076727 | |
| URL | - | |
| Relation | ||
| Gene | APX1 APX2 ATP6 DREB1A DREB1B PCL1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
