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Reference Detail
| Reference | ||
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| Author | Sun C., Wang Y., Yang X., Tang L., Wan C., Liu J., Chen C., Zhang H., He C., Liu C., Wang Q., Zhang K., Zhang W., Yang B., Li S., Zhu J., Sun Y., Li W., Zhou Y., Wang P., Deng X. | |
| Title | MATE transporter GFD1 cooperates with sugar transporters, mediates carbohydrate partitioning and controls grain-filling duration, grain size and number in rice. | |
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Abstract: More than half of the world's food is provided by cereals, as humans obtain >60% of daily calories from grains. Producing more carbohydrates is always the final target of crop cultivation. The carbohydrate partitioning pathway directly affects grain yield, but the molecular mechanisms and biological functions are poorly understood, including rice (Oryza sativa L.), one of the most important food sources. Here, we reported a prolonged grain filling duration mutant 1 (GFD1), exhibiting a long grain-filling duration, less grain number per panicle and bigger grain size without changing grain weight. Map-based cloning and molecular biological analyses revealed that GFD1 encoded a MATE transporter and expressed high in vascular tissues of the stem, spikelet hulls and rachilla, but low in the leaf, controlling carbohydrate partitioning in the stem and grain but not in the leaf. GFD1 protein was partially localized on the plasma membrane and in the Golgi apparatus, and was finally verified to interact with two sugar transporters, OsSWEET4 and OsSUT2. Genetic analyses showed that GFD1 might control grain-filling duration through OsSWEET4, adjust grain size with OsSUT2 and synergistically modulate grain number per panicle with both OsSUT2 and OsSWEET4. Together, our work proved that the three transporters, which are all initially classified in the major facilitator superfamily family, could control starch storage in both the primary sink (grain) and temporary sink (stem), and affect carbohydrate partitioning in the whole plant through physical interaction, giving a new vision of sugar transporter interactome and providing a tool for rice yield improvement. |
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| Journal | Plant Biotechnol. J. | |
| Country | China | |
| Volume | 21(3) | |
| Pages | 621-634 | |
| Year | 2023 | |
| PubMed ID | 36495424 | |
| PubMed Central ID | 9946139 | |
| DOI | 10.1111/pbi.13976 | |
| URL | - | |
| Relation | ||
| Gene | AGPL2 ALK FLO5 GFD1 SBE3 SSI SUT1 SUT2 SUT4 SUT5 SWEET13 SWEET4 SWEET5 WX1 XA13 XA41 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
