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Reference Detail
| Reference | ||
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| Author | Bakshi A., Moin M., Gayatri M.B., Reddy A.B.M., Datla R., Madhav M.S., Kirti P.B. | |
| Title | Involvement of Target of Rapamycin (TOR) Signaling in the Regulation of Crosstalk between Ribosomal Protein Small Subunit 6 Kinase-1 (RPS6K-1) and Ribosomal Proteins. | |
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Abstract: The target of rapamycin (TOR) protein phosphorylates its downstream effector p70kDa ribosomal protein S6 kinases (S6K1) for ribosome biogenesis and translation initiation in eukaryotes. However, the molecular mechanism of TOR-S6K1-ribosomal protein (RP) signaling is not well understood in plants. In the present study, we report the transcriptional upregulation of ribosomal protein large and small subunit (<i>RPL</i> and <i>RPS</i>) genes in the previously established <i>TOR</i> overexpressing transgenic lines of rice (in <i>Oryza sativa</i> ssp. <i>indica</i>, variety BPT-5204, TR-2.24 and TR-15.1) and of <i>Arabidopsis thaliana</i> (in Col 0 ecotype, ATR-1.4.27 and ATR-3.7.32). The mRNA levels of <i>RP</i> genes from this study were compared with those previously available in transcriptomic datasets on the expression of <i>RP</i>s in relation to TOR inhibitor and in the <i>TOR</i>-RNAi lines of <i>Arabidopsis thaliana</i>. We further analyzed TOR activity, i.e., S6K1 phosphorylation in SALK lines of Arabidopsis with mutation in <i>rpl6</i>, <i>rpl18</i>, <i>rpl23</i>, <i>rpl24</i> and <i>rps28C,</i> where the <i>rpl18</i> mutant showed inactivation of S6K1 phosphorylation. We also predicted similar putative Ser/Thr phosphorylation sites for ribosomal S6 kinases (RSKs) in the RPs of <i>Oryza sativa</i> ssp. <i>indica</i> and <i>Arabidopsis thaliana.</i> The findings of this study indicate that the TOR pathway is possibly interlinked in a cyclic manner via the phosphorylation of S6K1 as a modulatory step for the regulation of RP function to switch 'on'/'off' the translational regulation for balanced plant growth. |
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| Journal | Plants (Basel) | |
| Country | India | |
| Volume | 12(1) | |
| Pages | ||
| Year | 2023 | |
| PubMed ID | 36616305 | |
| PubMed Central ID | 9824793 | |
| DOI | 10.3390/plants12010176 | |
| URL | - | |
| Relation | ||
| Gene | BIP123 RPL18A RPL18A RPL23A RPL23E RPL30E RPL36.2 RPL38 RPL4 RPL51 RPL6 RPS25 RPS27 RPS28 RPS30 RPS3AC RPS6A RPS6E UBQ5 _ _ _ _ _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
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Stages in Each Organ - Muant Lines (Gene) |
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| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
