Update info.Tools - Reference - Detail
Reference Detail
| Reference | ||
|---|---|---|
| Author | Li Z., Liu J., Wang X., Wang J., Ye J., Xu S., Zhang Y., Hu D., Zhang M., Xu Q., Wang S., Yang Y., Wei X., Feng Y., Wang S. | |
| Title | <i>LG5</i>, a Novel Allele of <i>EUI1</i>, Regulates grain size and flag leaf Angle in Rice. | |
|
Abstract: grain size and flag leaf angle are two important traits that determining grain yield in rice. However, the mechanisms regulating these two traits remain largely unknown. In this study, a rice <i>long grain 5</i> (<i>lg5</i>) mutant with a large flag leaf angle was identified, and map-based cloning revealed that a single base substitution followed by a 2 bp insertion in the <i>LOC_Os05g40384</i> gene resulted in larger grains, a larger flag leaf angle, and higher plant height than the wild type. Sequence analysis revealed that <i>lg5</i> is a novel allele of <i>elongated uppermost internode-1</i> (<i>EUI1</i>), which encodes a cytochrome P450 protein. Functional complementation and overexpression tests showed that <i>LG5</i> can rescue the bigger grain size and larger flag leaf angle in the Xiushui11 (XS) background. Knockdown of the <i>LG5</i> transcription level by RNA interference resulted in elevated grain size and flag leaf angle in the Nipponbare (NIP) background. Morphological and cellular analyses suggested that <i>LG5</i> regulated grain size and flag leaf angle by promoting cell expansion and cell proliferation. Our results provided new insight into the functions of <i>EUI1</i> in rice, especially in regulating grain size and flag leaf angle, indicating a potential target for the improvement of rice breeding. |
||
| Journal | Plants (Basel) | |
| Country | China | |
| Volume | 12(3) | |
| Pages | ||
| Year | 2023 | |
| PubMed ID | 36771759 | |
| PubMed Central ID | 9921835 | |
| DOI | 10.3390/plants12030675 | |
| URL | - | |
| Relation | ||
| Gene | CDC2-2 CDKB2;1 CYCA3;2 CYCB1;1 CYCB2;2 EUI1 GS2 GS5 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
|
Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
