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Reference Detail
| Reference | ||
|---|---|---|
| Author | Li P., Jiang J., Zhang G., Miao S., Lu J., Qian Y., Zhao X., Wang W., Qiu X., Zhang F., Xu J. | |
| Title | Integrating GWAS and transcriptomics to identify candidate genes conferring heat tolerance in rice. | |
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Abstract: Rice (<i>Oryza sativa L.</i>) production is being challenged by global warming. Identifying new loci and favorable alleles associated with heat tolerance is crucial to developing rice heat-tolerant varieties. We evaluated the heat tolerance at the seedling stage using 620 diverse rice accessions. A total of six loci associated with heat tolerance were identified by a genome-wide association study (GWAS) with ~2.8 million single nucleotide polymorphisms (SNPs). Among the six detected loci, <i>qHT7</i> harbored the strongest association signal and the most associated SNPs. By comparing the transcriptomes of two representative accessions with contrasting heat tolerance, <i>LOC_Os07g48710</i> (<i>OsVQ30</i>) was selected as a promising candidate gene in <i>qHT7</i> due to the significant difference in its expression level between the two accessions. Haplotype 4 (Hap4) of <i>LOC_Os07g48710</i> was determined as the favorable haplotype for heat tolerance via the gene-based haplotype analysis. The heat-tolerant haplotype <i>LOC_Os07g48710</i>Hap4 is highly enriched in the tropical <i>Geng/Japonica</i> accessions, and its frequency has decreased significantly during the improvement process of rice varieties. Based on the GWAS and transcriptomics integrated results, a hypothetical model modulated by <i>qHT7</i> in response to heat stress was proposed. Our results provide valuable candidate genes for improving rice heat tolerance through molecular breeding. |
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| Journal | Front Plant Sci | |
| Country | China | |
| Volume | 13 | |
| Pages | 1102938 | |
| Year | 2022 | |
| PubMed ID | 36699845 | |
| PubMed Central ID | 9868562 | |
| DOI | 10.3389/fpls.2022.1102938 | |
| URL | - | |
| Relation | ||
| Gene | ACO3 APRL2 BIN2 COI1 CRL4 CYP735A4 ETR3 GID2 GRX2 GRX23 IAA31 JMT2 PDIL2-1 PDIL2-3 PDIL5-1 PDIL5-4 ROMT9 SAMT SGSD3 TARL1 VQ30 _ _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
