Tools - Reference - Detail

Reference Detail

Reference
Author Lu Q., Luo X., Yang X., Zhou T., Zhang Y., Lan Y., Zhang D., Zheng L., Li Y., Li L., Zhang S., Liu Y.
Title CRISPR/Cas9-mediated gene editing of <i>vacuolar ATPase subunit d</i> mediates phytohormone biosynthesis and virus resistance in rice.
Abstract:
Vacuolar ATPases (V-ATPases) are proton pumps for proton translocation across membranes that utilize energy derived from ATP hydrolysis; OsV-ATPase subunit d (OsV-ATPase d) is part of an integral, membrane-embedded V0 complex in the V-ATPase complex. Whether OsV-ATPase d is involved in phytohormone biosynthesis and resistance in rice remains unknown. The knockout mutants of <i>OsV-ATPase d</i> in rice were generated using the CRISPR/Cas9 system, and mutation of <i>OsV-ATPase d</i> did not show any detrimental effect on plant growth or yield productivity. Transcriptomic results showed that <i>OsV-ATPase d</i> is probably involved in mediating the biosynthesis of plant hormones and resistance in rice. Compared to wild type, mutation of <i>OsV-ATPase d</i> significantly increased JA and ABA biosynthesis and resistance against <i>Southern rice black-streaked dwarf virus</i> (SRBSDV), but it decreased resistance against <i>Rice stripe virus</i> (RSV) in rice. The data presented in this study reveal that <i>OsV-ATPase d</i> mediates phytohormone biosynthesis and virus resistance in rice and can be selected as a potential target for resistance breeding in rice.
Journal Front Plant Sci
Country China
Volume 14
Pages 1122978
Year 2023
PubMed ID 36818855
PubMed Central ID 9929465
DOI 10.3389/fpls.2023.1122978
URL -
Relation
Gene AOS3 CM-LOX1 NCED2 PAO7 SWEET15 TIFY5 VHA-D
INSD -
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain 		-
Stages in Each Organ
- Muant Lines (Gene) 		-
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase