Update info.Tools - Reference - Detail
Reference Detail
| Reference | ||
|---|---|---|
| Author | Huang Q., Lin B., Cao Y., Zhang Y., Song H., Huang C., Sun T., Long C., Liao J., Zhuo K. | |
| Title | CRISPR/Cas9-mediated mutagenesis of the susceptibility gene <i>OsHPP04</i> in rice confers enhanced resistance to rice root-knot nematode. | |
|
Abstract: CRISPR crops carrying a mutation in susceptibility (<i>S</i>) genes provide an effective strategy for controlling plant disease, because they could be 'transgene-free' and commonly have more broad-spectrum and durable type of resistance. Despite their importance, CRISPR/Cas9-mediated editing of <i>S</i> genes for engineering resistance to plant-parasitic nematode (PPN) disease has not been reported. In this study, we employed the CRISPR/Cas9 system to specifically induce targeted mutagenesis of the <i>S</i> gene rice copper metallochaperone heavy metal-associated plant protein 04 (<i>OsHPP04</i>), and successfully obtained genetically stable homozygous rice mutants with or without transgenic elements. These mutants confer enhanced resistance to the rice root-knot nematode (<i>Meloidogyne graminicola</i>), a major plant pathogenic nematode in rice agriculture. Moreover, the plant immune responses triggered by flg22, including reactive oxygen species burst, defence-related genes expression and callose deposition, were enhanced in the 'transgene-free' homozygous mutants. Analysis of rice growth and agronomic traits of two independent mutants showed that there are no obvious differences between wild-type plants and mutants. These findings suggest that <i>OsHPP04</i> may be an <i>S</i> gene as a negative regulator of host immunity and genetic modification of <i>S</i> genes through the CRISPR/Cas9 technology can be used as a powerful tool to generate PPN resistant plant varieties. |
||
| Journal | Front Plant Sci | |
| Country | China | |
| Volume | 14 | |
| Pages | 1134653 | |
| Year | 2023 | |
| PubMed ID | 36998699 | |
| PubMed Central ID | 10043372 | |
| DOI | 10.3389/fpls.2023.1134653 | |
| URL | - | |
| Relation | ||
| Gene | CYP709C9 EDS1 HPP4 KS4 PAL1 PR4 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
|
Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
