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Reference Detail
| Reference | ||
|---|---|---|
| Author | Jiang W., Ye Q., Wu Z., Zhang Q., Wang L., Liu J., Hu X., Guo D., Wang X., Zhang Z., He H., Hu L. | |
| Title | Analysis of CAT Gene Family and Functional Identification of OsCAT3 in Rice. | |
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Abstract: Catalase (CAT) is an important antioxidant enzyme in plants that plays a key role in plant growth and stress responses. CAT is usually encoded by a small gene family that has been cloned and functionally studied in some species, such as <i>Arabidopsis</i>, wheat and cucumber, but its specific roles in rice are not clear at present. In this study, we identified three CAT family genes (<i>OsCAT1</i>, <i>OsCAT2</i> and <i>OsCAT3</i>) in the rice genome and performed a systematic bioinformatics analysis. RT-PCR analysis revealed that <i>OsCAT1-OsCAT3</i> was primarily expressed in vegetative tissues such as roots, stems and leaves. Since <i>OsCAT3</i> showed the highest expression level among the three <i>OsCAT</i> genes, we then focused on its related functions. OsCAT3 prokaryotic expression protein has an obvious ability to remove H2O2. The <i>OsCAT3crispr</i> plant was short and had a low survival rate, the leaves were small with brown lesions, and the activities of the CAT, POD and SOD enzymes were significantly reduced. A microarray analysis showed that differentially expressed genes were primarily enriched in toxin metabolism and photosynthesis. This study laid a foundation for further understanding the function of the rice <i>OsCAT</i> gene. |
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| Journal | Genes (Basel) | |
| Country | China | |
| Volume | 14(1) | |
| Pages | ||
| Year | 2023 | |
| PubMed ID | 36672879 | |
| PubMed Central ID | 9858675 | |
| DOI | 10.3390/genes14010138 | |
| URL | - | |
| Relation | ||
| Gene | CATA CATB CPS2 CPS4 CYP99A3 KS4 KS7 KS8 KSL10 MAS NOE1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
