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Author Mo Y., Li G., Liu L., Zhang Y., Li J., Yang M., Chen S., Lin Q., Fu G., Zheng D., Ling Y.
Title <i>OsGRF4AA</i> compromises heat tolerance of developing pollen grains in rice.
Abstract:
Extreme high temperature at the meiosis stage causes a severe decrease in spikelet fertility and grain yield in rice. The rice variety grain size on chromosome 2 (GS2) contains sequence variations of <i>OsGRF4</i> (<i>Oryza sativa</i> growth-regulating factor 4; <i>OsGRF4AA</i> ), escaping the microRNA miR396-mediated degradation of this gene at the mRNA level. Accumulation of <i>OsGRF4</i> enhances nitrogen usage and metabolism, and increases grain size and grain yield. In this study, we found that pollen viability and seed-setting rate under heat stress (HS) decreased more seriously in GS2 than in its comparator, Zhonghua 11 (ZH11). Transcriptomic analysis revealed that, following HS, genes related to carbohydrate metabolic processes were expressed and regulated differentially in the anthers of GS2 and ZH11. Moreover, the expression of genes involved in chloroplast development and photosynthesis, lipid metabolism, and key transcription factors, including eight male sterile genes, were inhibited by HS to a greater extent in GS2 than in ZH11. Interestingly, pre-mRNAs of <i>OsGRF4</i>, and a group of essential genes involved in development and fertilization, were differentially spliced in the anthers of GS2 and ZH11. Taken together, our results suggest that variation in <i>OsGRF4</i> affects proper transcriptional and splicing regulation of genes under HS, and that this can be mediated by, and also feed back to, carbohydrate and nitrogen metabolism, resulting in a reduction in the heat tolerance of rice anthers.
Journal Front Plant Sci
Country China
Volume 14
Pages 1121852
Year 2023
PubMed ID 36909437
PubMed Central ID 9992635
DOI 10.3389/fpls.2023.1121852
URL -
Relation
Gene ABA8OX2 ABCG26 ABCG3 BHLH142 BHLH168 C6 CSA GELP45 GELP77 GRF1 GRF10 GRF11 GRF2 GRF3 GRF5 GRF6 GRF7 GRF8 GRF9 GS2 HSFA2A HSFB2C MIF1 MS2 PORA POX1 STRL2 TIP4;3 _ _ _
INSD -
Strain Wild Core Collection -
Induced Mutation Lines(NIG Collection) -
Sterile Seed Strain -
Lethal Embryo
Mutantion Strain
-
Stages in Each Organ
- Muant Lines (Gene)
-
Cultivated Varieties(NIG Collection) -
Stages in Each Organ -
/rice/oryzabase