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Reference Detail
| Reference | ||
|---|---|---|
| Author | Li Q., Zhai W., Wei J., Jia Y. | |
| Title | Rice lipid transfer protein, OsLTPL23, controls seed germination by regulating starch-sugar conversion and ABA homeostasis. | |
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Abstract: Seed germination is vital for ensuring the continuity of life in spermatophyte. High-quality seed germination usually represents good seedling establishment and plant production. Here, we identified OsLTPL23, a putative rice non-specific lipid transport protein, as an important regulator responsible for seed germination. Subcellular localization analysis confirmed that OsLTPL23 is present in the plasma membrane and nucleus. The knockout mutants of <i>OsLTPL23</i> were generated by CRISPR/Cas9-mediated genome editing, and <i>OsLTPL23</i> lines significantly germinated slower and lower than the Nipponbare (NIP). Starch and soluble sugar contents measurement showed that OsLTPL23 may have alpha-amylase inhibitor activity, and high soluble sugar content may be a causal agent for the delayed seed germination of <i>OsLTPL23</i> mutants. Transcript profiles in the germinating seeds exhibited that the abscisic acid (ABA)-responsive genes, <i>OsABI3</i> and <i>OsABI5</i>, and biosynthesis genes, <i>OsNCED1</i>, <i>OsNCED2</i>, <i>OsNCED3</i> and <i>OsNCED4</i>, are obviously upregulated in the <i>OsLTPL23</i> mutants compared to NIP plants, conversely, ABA metabolism genes <i>OsABA8ox1</i>, <i>OsABA8ox2</i> and <i>OsABA8ox3</i> are stepwise decreased. Further investigations found that <i>OsLTPL23</i> mutants displays weakened early seedling growth, with elevated gene expresssion of ABA catabolism genes and repressive transcription response of defence-related genes <i>OsWRKY45, OsEiN3, OsPR1a</i>, <i>OsPR1b</i> and <i>OsNPR1</i>. Integrated analysis indicated that <i>OsLTPL23</i> may exert an favorable effect on rice seed germination and early seedling growth <i>via</i> modulating endogenous ABA homeostasis. Collectively, our study provides important insights into the roles of <i>OsLTPL23</i>-mediated carbohydrate conversion and endogenous ABA pathway on seed germination and early seedling growth, which contributes to high-vigor seed production in rice breeding. |
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| Journal | Front Genet | |
| Country | China | |
| Volume | 14 | |
| Pages | 1111318 | |
| Year | 2023 | |
| PubMed ID | 36726806 | |
| PubMed Central ID | 9885049 | |
| DOI | 10.3389/fgene.2023.1111318 | |
| URL | - | |
| Relation | ||
| Gene | ABA8OX1 ABA8OX2 ABA8OX3 ABI5 BHLH9 LTP1.15 NCED1 NCED1 NCED2 NCED3 NH1 PR1A PR1B VP1 WRKY45 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
