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Reference Detail
| Reference | ||
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| Author | Patel A., Sahu K.P., Mehta S., Balamurugan A., Kumar M., Sheoran N., Kumar S., Krishnappa C., Ashajyothi M., Kundu A., Goyal T., Narayanasamy P., Kumar A. | |
| Title | Rice leaf endophytic <i>Microbacterium testaceum</i>: Antifungal actinobacterium confers immunocompetence against rice blast disease. | |
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Abstract: Genetic and functional characteristics of rice leaf endophytic actinobacterial member, <i>Microbacterium</i> are described. Morphotyping, multilocus sequence analysis and transmission electron microscopy indicated the species identity of the endophytic bacterium, OsEnb-ALM-D18, as <i>Microbacterium testaceum</i>. The endophytic <i>Microbacterium</i> showed probiotic solubilization of plant nutrients/minerals, produced hydrolytic enzyme/phytohormones, and showed endophytism in rice seedlings. Further, the endophytic colonization by <i>M. testaceum</i> OsEnb-ALM-D18 was confirmed using reporter gene coding for green fluorescence protein. <i>Microbacterium</i> OsEnb-ALM-D18 showed volatilome-mediated antibiosis (95.5% mycelial inhibition) on <i>Magnaporthe oryzae</i>. Chemical profiling of <i>M. testaceum</i> OsEnb-ALM-D18 volatilome revealed the abundance of 9-Octadecenoic acid, Hexadecanoic acid, 4-Methyl-2-pentanol, and 2,5-Dihydro-thiophene. Upon endobacterization of rice seedlings, <i>M. testaceum</i> altered shoot and root phenotype suggestive of activated defense. Over 80.0% blast disease severity reduction was observed on the susceptible rice cultivar Pusa Basmati-1 upon foliar spray with <i>M. testaceum</i>. qPCR-based gene expression analysis showed induction of <i>Os</i>CERK1, <i>Os</i>PAD4, <i>Os</i>NPR1.3, and <i>Os</i>FMO1 suggestive of endophytic immunocompetence against blast disease. Moreover, <i>M. testaceum</i> OsEnb-ALM-D18 conferred immunocompetence, and antifungal antibiosis can be the future integrated blast management strategy. |
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| Journal | Front Microbiol | |
| Country | India | |
| Volume | 13 | |
| Pages | 1035602 | |
| Year | 2022 | |
| PubMed ID | 36619990 | |
| PubMed Central ID | 9810758 | |
| DOI | 10.3389/fmicb.2022.1035602 | |
| URL | - | |
| Relation | ||
| Gene | CEBIP CERK1 EDS1 NH2 PAD4 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
