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Reference Detail
| Reference | ||
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| Author | Kamara N., Lu Z., Jiao Y., Zhu L., Wu J., Chen Z., Wang L., Liu X., Shahid M.Q. | |
| Title | An uncharacterized protein NY1 targets EAT1 to regulate anther tapetum development in polyploid rice. | |
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Abstract: Autotetraploid rice is a useful germplasm for the breeding of polyploid rice; however, low fertility is a major hindrance for its utilization. Neo-tetraploid rice with high fertility was developed from the crossing of different autotetraploid rice lines. Our previous research showed that the mutant (NY1) of LOC_Os07g32406 (NY1), which was generated by CRISPR/Cas9 knock-out in neo-tetraploid rice, showed low pollen fertility, low seed set, and defective chromosome behavior during meiosis. However, the molecular genetic mechanism underlying the fertility remains largely unknown. Here, cytological observations of the NY1 mutant (NY1) indicated that NY1 exhibited abnormal tapetum and middle layer development. RNA-seq analysis displayed a total of 5606 differentially expressed genes (DEGs) in NY1 compared to wild type (H1) during meiosis, of which 2977 were up-regulated and 2629 were down-regulated. Among the down-regulated genes, 16 important genes associated with tapetal development were detected, including EAT1, CYP703A3, CYP704B2, DPW, PTC1, OsABCG26, OsAGO2, SAW1, OsPKS1, OsPKS2, and OsTKPR1. The mutant of EAT1 was generated by CRISPR/Cas9 that showed abnormal tapetum and pollen wall formation, which was similar to NY1. Moreover, 478 meiosis-related genes displayed down-regulation at same stage, including 9 important meiosis-related genes, such as OsREC8, OsSHOC1, SMC1, SMC6a and DCM1, and their expression levels were validated by qRT-PCR. Taken together, these results will aid in identifying the key genes associated with pollen fertility, which offered insights into the molecular mechanism underlying pollen development in tetraploid rice. |
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| Journal | BMC Plant Biol. | |
| Country | China | |
| Volume | 22(1) | |
| Pages | 582 | |
| Year | 2022 | |
| PubMed ID | 36514007 | |
| PubMed Central ID | 9746164 | |
| DOI | 10.1186/s12870-022-03976-0 | |
| URL | - | |
| Relation | ||
| Gene | ABCG26 AGO2 CYP703A3 CYP704B2 DCM1 DFR2 EAT1 HXK7 HXK9 LAP6 MS2 NY1 PKS10 PTC1 RAD21-4 RSUS2 RSUS3 SHOC1 SMC1 SMC6 SUT1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
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Stages in Each Organ - Muant Lines (Gene) |
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| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
