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Reference Detail
| Reference | ||
|---|---|---|
| Author | Mei E., Tang J., He M., Liu Z., Tian X., Bu Q. | |
| Title | OsMKKK70 Negatively Regulates cold tolerance at Booting Stage in Rice. | |
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Abstract: Cold stress at the booting stage leads to a lower seed setting rate and seriously threatens the production of rice (<i>Oryza sativa</i> L.), which has become a major yield-limiting factor in higher-altitude and -latitude regions. Because cold tolerance at the booting stage (CTB) is a complex trait and is controlled by multiple loci, only a few genes have been reported so far. In this study, a function of OsMKKK70 (Mitogen Activated Protein Kinase Kinase Kinase 70) in response to CTB was characterized. <i>OsMKKK70</i> expression was rapidly induced by cold stress at the booting stage. <i>OsMKKK70</i> overexpression (<i>OsMKKK70-OE</i>) plants were more sensitive to cold stress at the booting stage with a lower seed setting and pollen fertility, but there was no significant difference between the <i>OsMKKK70</i> mutant and WT. Considering the effect of functional redundancy, we further tested the CTB response of <i>OsMKKK62/70</i> and <i>OsMKKK55/62/70</i>, the double and triple mutants of <i>OsMKKK70</i> with its closest homologs <i>OsMKKK62</i> and <i>OsMKKK55</i>, and found that <i>OsMKKK62/70</i> and <i>OsMKKK55/62/70</i> displayed significantly increased CTB with a higher seed setting and pollen fertility, indicating that OsMKKK70 negatively regulates rice CTB. Moreover, under the low-temperature (LT) condition, the <i>OsMKKK62/70</i> mutant had slightly higher Gibberellin (GA) contents, increased expression of GA biosynthesis genes, and lower protein level of OsSLR1 in anthers than those in WT. By contrast, <i>OsMKKK70-OE</i> anther had a lower GA biosynthesis than that of WT. Together, these findings suggest that OsMKKK70 negatively regulates rice CTB by fine-tuning GA levels in anthers. |
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| Journal | Int J Mol Sci | |
| Country | China | |
| Volume | 23(22) | |
| Pages | ||
| Year | 2022 | |
| PubMed ID | 36430953 | |
| PubMed Central ID | 9697274 | |
| DOI | 10.3390/ijms232214472 | |
| URL | - | |
| Relation | ||
| Gene | CYP703A3 GA20OX3 GA3OX1 GAMYB GNP1 MAP3K6 SLR1 STE11 WRKY53 _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
