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Reference Detail
| Reference | ||
|---|---|---|
| Author | Xu T., Zhang J., Liu Y., Zhang Q., Li W., Zhang Y., Wu M., Chen T., Ding D., Wang W., Zhang Z. | |
| Title | Exon skipping in <i>IspE</i> Gene is associated with abnormal chloroplast development in rice <i>albino leaf 4</i> mutant. | |
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Abstract: The formation of leaf color largely depends on the components of pigment accumulation in plastids, which are involved in chloroplast development and division. Here, we isolated and characterized the rice <i>albino leaf 4</i> (<i>al4</i>) mutant, which exhibited an albino phenotype and eventually died at the three-leaf stage. The chloroplasts in <i>al4</i> mutant were severely damaged and unable to form intact thylakoid structure. Further analysis revealed that the candidate gene encodes 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (IspE), which participates in the methylerythritol phosphate (MEP) pathway of isoprenoid biosynthesis. We further demonstrated that the mutation at the exon-intron junction site cause alternative splicing factors fail to distinguish the origin of the GT-AG intron, leading to exon skipping and producing a truncated <i>OsIspE</i> in the <i>al4</i> mutant. Notably, disruption of <i>OsIspE</i> led to the reduced expression of chloroplast-associated genes, including chloroplast biosynthetic and translation related genes and photosynthetic associated nuclear genes (PhANGs). In summary, these findings reveal that <i>OsIspE</i> plays a crucial role in chloroplast biogenesis and provides novel insights into the function of CMK during chloroplast development in rice. |
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| Journal | Front Plant Sci | |
| Country | China | |
| Volume | 13 | |
| Pages | 986678 | |
| Year | 2022 | |
| PubMed ID | 36426160 | |
| PubMed Central ID | 9678938 | |
| DOI | 10.3389/fpls.2022.986678 | |
| URL | - | |
| Relation | ||
| Gene | CAB1R GRY340 HAP3A LHCB2.1 LSD3 NAD2 NAD5 PETA PSAD PSAE PSBA PSBO PSBP RBCS3 YGL10 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
