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Reference Detail
| Reference | ||
|---|---|---|
| Author | Prodhan Z.H., Islam S.A., Alam M.S., Li S., Jiang M., Tan Y., Shu Q. | |
| Title | Impact of <i>OsBadh2</i> Mutations on Salt Stress Response in Rice. | |
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Abstract: Mutations in the <i>Betaine aldehyde dehydrogenase 2</i> (<i>OsBadh2</i>) gene resulted in aroma, which is a highly preferred grain quality attribute in rice. However, research on naturally occurring aromatic rice has revealed ambiguity and controversy regarding aroma emission, stress tolerance, and response to salinity. In this study, mutant lines of two non-aromatic varieties, Huaidao#5 (WT_HD) and Jiahua#1 (WT_JH), were generated by targeted mutagenesis of <i>OsBadh2</i> using CRISPR/Cas9 technology. The mutant lines of both varieties became aromatic; however, WT_HD mutants exhibited an improved tolerance, while those of WT_JH showed a reduced tolerance to salt stress. To gain insight into the molecular mechanism leading to the opposite effects, comparative analyses of the physiological activities and expressions of aroma- and salinity-related genes were investigated. The WT_HD mutants had a lower mean increment rate of malondialdehyde, superoxide dismutase, glutamate, and proline content, with a higher mean increment rate of gamma-aminobutyric acid, hydrogen peroxide, and catalase than the WT_JH mutants. Fluctuations were also detected in the salinity-related gene expression. Thus, the response mechanism of <i>OsBadh2</i> mutants is complicated where the genetic makeup of the rice variety and interactions of several genes are involved, which requires more in-depth research to explore the possibility of producing highly tolerant aromatic rice genotypes. |
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| Journal | Plants (Basel) | |
| Country | China | |
| Volume | 11(21) | |
| Pages | ||
| Year | 2022 | |
| PubMed ID | 36365282 | |
| PubMed Central ID | 9656462 | |
| DOI | 10.3390/plants11212829 | |
| URL | - | |
| Relation | ||
| Gene | ALDH18B2 BAD1 DREB1C ERF68 GAD1 GAD2 GAD3 GAD4 HAK5 MYB30 NAC3 P5CS SK2(T)(SCL, FGR) | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
