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Reference Detail
| Reference | ||
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| Author | Mun B.G., Shahid M., Lee G.S., Hussain A., Yun B.W. | |
| Title | A Novel rhs1 Locus in Rice Attributes Seed-Pod SHATTERING by the Regulation of Endogenous S-Nitrosothiols. | |
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Abstract: Seed or pod SHATTERING in rice (<i>Oryza</i> sativa) is considered to be one of the major factors involved in the domestication of rice as a crop. High seed SHATTERING results in significant yield losses. In this study, we characterize the <i>RICE</i><i>HIGH</i><i>SHATTERING 1</i> (<i>rhs1</i>) that corresponds to the locus <i>LOC_Os04g41250</i> from a greenhouse screen, involving 145 Ac/Ds transposon mutant rice lines. The knockout mutant line <i>rhs1</i> exhibited a significantly high SHATTERING of grains in comparison to the wild-type plants. The exogenous application of nitric oxide (NO) resulted in a significant reduction in the expression of <i>rhs1</i> in wild-type rice plants. The absence of <i>rhs1</i>, which encodes a putative armadillo/beta-catenin repeat family protein, resulted in high sensitivity of the <i>rhs1</i> plants to nitrosative stress. Interestingly, the basal expression levels of <i>QSH1</i> and <i>SHAT1</i> genes (transcription factors that regulate seed-pod SHATTERING in rice) were significantly lower in these plants than in wild-type plants; however, nitrosative stress negatively regulated the expression of <i>QSH1</i> and <i>SHAT1</i> in both WT and <i>rhs1</i> plants, but positively regulated <i>QSH4</i> expression in <i>rhs1</i> plants alone. The expression levels of genes responsible for NO production (<i>OsNIA1</i>, <i>OsNIA2,</i> and <i>OsNOA1</i>) were lower in <i>rhs1</i> plants than in WT plants under normal conditions. However, under nitrosative stress, the expression of <i>OsNIA2</i> significantly increased in <i>rhs1</i> plants. The expression of <i>CPL1</i> (a negative regulator of seed SHATTERING in rice) was significantly lower in <i>rhs1</i> plants, and we found that <i>CPL1</i> expression was correlated with <i>S</i>-nitrosothiol (SNO) alteration in <i>rhs1.</i> Interestingly <i>noe1</i>, a rice mutant with high SNO levels, exhibited low seed SHATTERING, whereas <i>rhs1</i> resulted in low SNO levels with high seed SHATTERING. Therefore, <i>rhs1</i> is a novel gene that negatively regulates the SHATTERING trait in rice via regulation of endogenous SNO levels. However, the molecular mechanisms involved in the control of <i>rhs1</i>-mediated regulation of seed SHATTERING and its interaction with nitric oxide and involvement in plant defense need to be investigated further. |
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| Journal | Int J Mol Sci | |
| Country | South Korea | |
| Volume | 23(21) | |
| Pages | ||
| Year | 2022 | |
| PubMed ID | 36362013 | |
| PubMed Central ID | 9655508 | |
| DOI | 10.3390/ijms232113225 | |
| URL | - | |
| Relation | ||
| Gene | GSNOR1 NOA1 NOE1 QSH1 RHS1 SH-H SH4 SHAT1 _ _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
