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Reference Detail
| Reference | ||
|---|---|---|
| Author | Zhan J., Zou W., Li S., Tang J., Lu X., Meng L., Ye G. | |
| Title | OsNAC15 Regulates Tolerance to Zinc Deficiency and Cadmium by Binding to <i>OsZIP7</i> and <i>OsZIP10</i> in Rice. | |
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Abstract: Zinc (Zn) deficiency and cadmium (Cd) stress are severe threats to the growth and development of plants. Increasing Zn content and/or decreasing Cd content in grain are also important objectives of rice breeding. However, the molecular mechanisms of Zn deficiency tolerance (ZDT) and Cd stress tolerance (CDT) are largely unknown in rice. Here, we report that a NAM/CUC2-like transcription factor, <i>OsNAC15</i>, contributes to ZDT and CDT in rice. Knockout of <i>OsNAC15</i> reduced ZDT and CDT at the vegetative stage. <i>OsNAC15</i> expresses in all tissues of different developmental stages, and is repressed by Zn deficiency and induced by Cd stress. OsNAC15 is a functional transcription factor with transactivation and DNA binding activities. Expression analysis of rice ZIP family genes suggested that the knockout of <i>OsNAC15</i> activates or inhibits their transcriptions under Zn deficiency or Cd stress conditions. The yeast one-hybrid assay, transient transcriptional activity assay using the dual-luciferase reporter system and electrophoretic mobility shift assay demonstrated that OsNAC15 directly binds to the zinc deficiency-responsive element motifs in the promoters of <i>OsZIP7</i> and <i>OsZIP10</i> to repress their transcriptions. The <i>OsNAC15-OsZIP7/10</i> module is an essential foundation for further study on the regulatory mechanisms of ZDT and CDT in rice. |
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| Journal | Int J Mol Sci | |
| Country | China | |
| Volume | 23(19) | |
| Pages | ||
| Year | 2022 | |
| PubMed ID | 36233067 | |
| PubMed Central ID | 9569620 | |
| DOI | 10.3390/ijms231911771 | |
| URL | - | |
| Relation | ||
| Gene | IRT1 IRT2 NAC15 ZIP1 ZIP10 ZIP11 ZIP13 ZIP14 ZIP16 ZIP2 ZIP3 ZIP4 ZIP5 ZIP6 ZIP7 ZIP8 _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
