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Reference Detail
| Reference | ||
|---|---|---|
| Author | Nguyen N.K., Wang J., Liu D., Hwang B.K., Jwa N.S. | |
| Title | Rice iron storage protein Ferritin 2 (OsFER2) positively regulates ferroptotic cell death and defense responses against <i>Magnaporthe oryzae</i>. | |
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Abstract: Ferritin is a ubiquitous iron storage protein that regulates iron homeostasis and oxidative stress in plants. Iron plays an important role in ferroptotic cell death response of rice (<i>Oryza sativa</i>) to <i>Magnaporthe oryzae</i> infection. Here, we report that rice Ferritin 2, OsFER2, is required for iron- and reactive oxygen species (ROS)-dependent ferroptotic cell death and defense response against the avirulent <i>M. oryzae</i> INA168. The full-length Ferritin OsFER2 and its transit peptide were localized to the chloroplast, the most Fe-rich organelle for photosynthesis. This suggests that the transit peptide acts as a signal peptide for the rice Ferritin OsFER2 to move into chloroplasts. <i>OsFER2</i> expression is involved in rice resistance to <i>M. oryzae</i> infection. <i>OsFER2</i> knock-out in wild-type rice HY did not induce ROS and ferric ion (Fe3+) accumulation, lipid peroxidation and hypersensitive response (HR) cell death, and also downregulated the defense-related genes <i>OsPAL1</i>, <i>OsPR1-b</i>, <i>OsRbohB</i>, <i>OsNADP-ME2-3</i>, <i>OsMEK2</i> and <i>OsMPK1</i>, and vacuolar membrane transporter <i>OsVIT2</i> expression. <i>OsFER2</i> complementation in <i>DeltaOsfer2</i> knock-out mutants restored ROS and iron accumulation and HR cell death phenotypes during infection. The iron chelator deferoxamine, the lipid-ROS scavenger ferrostatin-1, the actin microfilament polymerization inhibitor cytochalasin E and the redox inhibitor diphenyleneiodonium suppressed ROS and iron accumulation and HR cell death in rice leaf sheaths. However, the small-molecule inducer erastin did not trigger iron-dependent ROS accumulation and HR cell death induction in <i>DeltaOsfer2</i> mutants. These combined results suggest that <i>OsFER2</i> expression positively regulates iron- and ROS-dependent ferroptotic cell death and defense response in rice-<i>M. oryzae</i> interactions. |
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| Journal | Front Plant Sci | |
| Country | South Korea | |
| Volume | 13 | |
| Pages | 1019669 | |
| Year | 2022 | |
| PubMed ID | 36352872 | |
| PubMed Central ID | 9639352 | |
| DOI | 10.3389/fpls.2022.1019669 | |
| URL | - | |
| Relation | ||
| Gene | DSG1 FER2 MKK1 NADP-ME2 RBOHB VIT1 _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
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Stages in Each Organ - Muant Lines (Gene) |
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| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
