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Reference Detail
| Reference | ||
|---|---|---|
| Author | Kim S.H., Shim K.C., Lee H.S., Jeon Y.A., Adeva C., Luong N.H., Ahn S.N. | |
| Title | Brassinosteroid biosynthesis gene <i>OsD2</i> is associated with low-temperature germinability in rice. | |
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Abstract: In rice, low-temperature germinability (LTG) is essential for stable stand establishment using the direct seeding method in temperate and high-altitude areas. Previously, we reported that the quantitative trait locus <i>qLTG1</i> is associated with LTG. <i>qLTG1</i> is also associated with tolerance to several abiotic stresses, such as salt and osmotic conditions. In this study, map-based cloning and sequence analysis indicated that <i>qLTG1</i> is allelic to <i>DWARF2</i> (<i>OsD2</i>), which encodes cytochrome P450 D2 (LOC_Os01g10040) involved in brassinosteroid (BR) biosynthesis. Sequence comparison of the two parental lines, Hwaseong and <i>Oryza rufipogon</i> (IRGC 105491), revealed five single nucleotide polymorphisms (SNPs) in the coding region. Three of these SNPs led to missense mutations in <i>OsD2</i>, whereas the other two SNPs were synonymous. We evaluated two T-DNA insertion mutants, <i>viz.</i>, overexpression (<i>OsD2</i>-OE) and knockdown (<i>OsD2</i>-KD) mutants of <i>OsD2</i>, with the Dongjin genetic background. <i>OsD2</i>-KD plants showed a decrease in LTG and grain size. In contrast, <i>OsD2</i>-OE plants showed an increase in grain size.and LTG. We also examined the expression levels of several BR signaling and biosynthetic genes using the T-DNA insertion mutants. Gene expression analysis and BR application experiments demonstrated that BR enhanced the seed germination rate under low-temperature conditions. These results suggest that <i>OsD2</i> is associated with the regulation of LTG and improving grain size. Thus, <i>OsD2</i> may be a suitable target for rice breeding programs to improve rice yield and LTG. |
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| Journal | Front Plant Sci | |
| Country | South Korea | |
| Volume | 13 | |
| Pages | 985559 | |
| Year | 2022 | |
| PubMed ID | 36204076 | |
| PubMed Central ID | 9530605 | |
| DOI | 10.3389/fpls.2022.985559 | |
| URL | - | |
| Relation | ||
| Gene | BIN2 BRD1 BU1 BZR1 CPD1 D11 D2 D61 DLT GSK4 SG2 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
