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Reference Detail
| Reference | ||
|---|---|---|
| Author | Ninkuu V., Yan J., Zhang L., Fu Z., Yang T., Li S., Li B., Duan J., Ren J., Li G., Yang X., Zeng H. | |
| Title | Hrip1 mediates rice cell wall fortification and phytoalexins elicitation to confer immunity against <i>Magnaporthe oryzae</i>. | |
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Abstract: <i>Magnaporthe oryzae</i> is a potent fungus that adversely affects rice yield. Combinatorial techniques of prevention, toxic chemicals, and fungicide are used to remedy rice blast infection. We reported the role of Hrip1 in cell death elicitation and expression of systematic acquired resistance that could potentially stifle <i>M. oryzae</i> infection. In this study, transcriptome and metabolomic techniques were used to investigate the mechanism by which Hrip1 reprogramed the transcriptome of rice seedlings to confer immunity against <i>M. oryzae</i>. Our results showed that Hrip1 induces cell wall thickening and phytoalexin elicitation to confer immunity against <i>M. oryzae</i> infection. Hrip1 activates key lignin biosynthetic genes and myeloblastosis transcription factors that act as molecular switches for lignin production. Lignin content was increased by 68.46% and more after 48 h onwards in Hrip1-treated seedlings compared to the control treatment. Further analysis of cell wall morphology using the transmission electron microscopy technique revealed over 100% cell wall robustness. Hrip1 also induced the expression of 24 diterpene synthases. These include class I and II terpene synthases, cytochrome P450 subfamilies (OsCYP76M and OsCYP71Z), and momilactones synthases. The relationship between the expression of these genes and metabolic elicitation was analyzed using ultra-performance liquid chromatography-tandem mass spectrometry. Enhanced amounts of momilactones A and B, oryzalactone, and phytocassane A and G were detected in the Hrip1-treated leaves. We also identified seven benzoxazinoid genes (BX1-BX7) that could improve rice immunity. Our findings show that Hrip1 confers dual immunity by leveraging lignin and phytoalexins for physical and chemical resistance. This study provides novel insights into the mechanisms underlying Hrip1-treated plant immunity. |
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| Journal | Front Plant Sci | |
| Country | China | |
| Volume | 13 | |
| Pages | 980821 | |
| Year | 2022 | |
| PubMed ID | 36212323 | |
| PubMed Central ID | 9546723 | |
| DOI | 10.3389/fpls.2022.980821 | |
| URL | - | |
| Relation | ||
| Gene | 2ODD23 4CL5 ASMT1 C4H2 CCR21 CPS2 CPS4 CYP71C1 CYP71X5 CYP71Z2 CYP71Z21 CYP71Z7 CYP76M14 CYP76M2 CYP76M5 CYP76M6 CYP76M7 CYP76M8 GGPPS2 IGL KS4 KS7 KS8 KSL10 MAS NOMT PRX39 SDR110C-MI3 SDR110C-MS2 SDR110C-MS3 SDR7-6 THT1 TS2 _ _ _ _ _ _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
|
Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
