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Reference Detail
| Reference | ||
|---|---|---|
| Author | Chen Y., Wang J., Nguyen N.K., Hwang B.K., Jwa N.S. | |
| Title | The NIN-Like Protein OsNLP2 Negatively Regulates Ferroptotic Cell Death and Immune Responses to <i>Magnaporthe oryzae</i> in Rice. | |
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Abstract: Nodule inception (NIN)-like proteins (NLPs) have a central role in nitrate signaling to mediate plant growth and development. Here, we report that OsNLP2 negatively regulates ferroptotic cell death and immune responses in rice during <i>Magnaporthe oryzae</i> infection. OsNLP2 was localized to the plant cell nucleus, suggesting that it acts as a transcription factor. OsNLP2 expression was involved in susceptible disease development. <i>DeltaOsnlp2</i> knockout mutants exhibited reactive oxygen species (ROS) and iron-dependent ferroptotic hypersensitive response (HR) cell death in response to <i>M. oryzae</i>. Treatments with the iron chelator deferoxamine, lipid-ROS scavenger ferrostatin-1, actin polymerization inhibitor cytochalasin A, and NADPH oxidase inhibitor diphenyleneiodonium suppressed the accumulation of ROS and ferric ions, lipid peroxidation, and HR cell death, which ultimately led to successful <i>M. oryzae</i> colonization in <i>DeltaOsnlp2</i> mutants. The loss-of-function of OsNLP2 triggered the expression of defense-related genes including <i>OsPBZ1</i>, <i>OsPIP-3A</i>, <i>OsWRKY104</i>, and <i>OsRbohB</i> in <i>DeltaOsnlp2</i> mutants. <i>DeltaOsnlp2</i> mutants exhibited broad-spectrum, nonspecific resistance to diverse <i>M. oryzae</i> strains. These combined results suggest that OsNLP2 acts as a negative regulator of ferroptotic HR cell death and defense responses in rice, and may be a valuable gene source for molecular breeding of rice with broad-spectrum resistance to blast disease. |
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| Journal | Antioxidants (Basel) | |
| Country | South Korea | |
| Volume | 11(9) | |
| Pages | ||
| Year | 2022 | |
| PubMed ID | 36139868 | |
| PubMed Central ID | 9495739 | |
| DOI | 10.3390/antiox11091795 | |
| URL | - | |
| Relation | ||
| Gene | NLP2 PIP2;7 PR10A RBOHB WRKY104 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
