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Reference Detail
| Reference | ||
|---|---|---|
| Author | Liu R., Feng Q., Li P., Lou G., Chen G., Jiang H., Gao G., Zhang Q., Xiao J., Li X., Xiong L., He Y. | |
| Title | <i>GLW7.1</i>, a Strong Functional Allele of <i>Ghd7</i>, Enhances grain size in Rice. | |
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Abstract: grain size is a key determinant of both grain weight and grain quality. Here, we report the map-based cloning of a novel quantitative trait locus (QTL), <i>GLW7.1</i> (<i>Grain Length</i>, <i>Width and Weight 7.1</i>), which encodes the CCT motif family protein, GHD7. The QTL is located in a 53 kb deletion fragment in the cultivar Jin23B, compared with the cultivar CR071. Scanning electron microscopy analysis and expression analysis revealed that <i>GLW7.1</i> promotes the transcription of several cell division and expansion genes, further resulting in a larger cell size and increased cell number, and finally enhancing the grain size as well as grain weight. <i>GLW7.1</i> could also increase endogenous GA content by up-regulating the expression of GA biosynthesis genes. Yeast two-hybrid assays and split firefly luciferase complementation assays revealed the interactions of GHD7 with seven grain-size-related proteins and the rice DELLA protein SLR1. Haplotype analysis and transcription activation assay revealed the effect of six amino acid substitutions on GHD7 activation activity. Additionally, the NIL with <i>GLW7.1</i> showed reduced chalkiness and improved cooking and eating quality. These findings provide a new insight into the role of <i>Ghd7</i> and confirm the great potential of the <i>GLW7.1</i> allele in simultaneously improving grain yield and quality. |
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| Journal | Int J Mol Sci | |
| Country | China | |
| Volume | 23(15) | |
| Pages | ||
| Year | 2022 | |
| PubMed ID | 35955848 | |
| PubMed Central ID | 9369204 | |
| DOI | 10.3390/ijms23158715 | |
| URL | - | |
| Relation | ||
| Gene | FBOX130 FZP GHD7 HAP5L NAC24 NAC25 RSR1 SLR1 SNB | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
