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Reference Detail
| Reference | ||
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| Author | Cai Y., Zhang W., Fu Y., Shan Z., Xu J., Wang P., Kong F., Jin J., Yan H., Ge X., Wang Y., You X., Chen J., Li X., Chen W., Chen X., Ma J., Tang X., Zhang J., Bao Y., Jiang L., Wang H., Wan J. | |
| Title | Du13 encodes a C2 H2 zinc-finger protein that regulates Wxb pre-mRNA splicing and microRNA biogenesis in rice endosperm. | |
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Abstract: amylose content is a crucial physicochemical property responsible for the eating and cooking quality of rice (Oryza sativa L.) grain and is mainly controlled by the Waxy (Wx) gene. Previous studies have identified several Dull genes that modulate the expression of the Wxb allele in japonica rice by affecting the splicing efficiency of the Wxb pre-mRNA. Here, we uncover dual roles for a novel Dull gene in pre-mRNA splicing and microRNA processing. We isolated the dull mutant, Du13, with a dull endosperm and low amylose content. Map-based cloning showed that Du13 encodes a C2 H2 zinc-finger protein. Du13 coordinates with the nuclear cap-binding complex to regulate the splicing of Wxb transcripts in rice endosperm. Moreover, Du13 also regulates alternative splicing of other protein-coding transcripts and affects the biogenesis of a subset of microRNAs. Our results reveal an evolutionarily conserved link between pre-mRNA splicing and microRNA biogenesis in rice endosperm. Our findings also provide new insights into the functions of Dull genes in rice and expand our knowledge of microRNA biogenesis in monocots. |
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| Journal | Plant Biotechnol. J. | |
| Country | China | |
| Volume | 20(7) | |
| Pages | 1387-1401 | |
| Year | 2022 | |
| PubMed ID | 35560858 | |
| PubMed Central ID | 9241381 | |
| DOI | 10.1111/pbi.13821 | |
| URL | - | |
| Relation | ||
| Gene | CBP80 DU13 DU3 HYL1 MIR1850 MIR1866 MIR435 MIR444E RSP29 RSZP21A RSZP21B RSZP23 WX1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
