Update info.Tools - Reference - Detail
Reference Detail
| Reference | ||
|---|---|---|
| Author | Liao Y., Ali A., Xue Z., Zhou X., Ye W., Guo D., Liao Y., Jiang P., Wu T., Zhang H., Xu P., Chen X., Zhou H., Liu Y., Wang W., Wu X. | |
| Title | Disruption of <i>LLM9428</i>/<i>OsCATC</i> Represses Starch Metabolism and Confers Enhanced Blast Resistance in Rice. | |
|
Abstract: Catalases (CATs) are important self-originating enzymes and are involved in many of the biological functions of plants. Multiple forms of CATs suggest their versatile role in lesion mimic mutants (LMMs), H2O2 homeostasis and abiotic and biotic stress tolerance. In the current study, we identified a <i>large lesion mimic mutant9428</i> (<i>llm9428</i>) from Ethyl-methane-sulfonate (EMS) mutagenized population. The <i>llm9428</i> showed a typical phenotype of LMMs including decreased agronomic yield traits. The histochemical assays showed decreased cell viability and increased reactive oxygen species (ROS) in the leaves of <i>llm9428</i> compared to its wild type (WT). The <i>llm9428</i> showed enhanced blast disease resistance and increased relative expression of pathogenesis-related (PR) genes. Studies of the sub-cellular structure of the leaf and quantification of starch contents revealed a significant decrease in starch granule formation in <i>llm9428</i>. Genetic analysis revealed a single nucleotide change (C > T) that altered an amino acid (Ala > Val) in the candidate gene (<i>Os03g0131200</i>) encoding a CATALASE C in <i>llm9428</i>. CRISPR-Cas9 targetted knockout lines of <i>LLM9428/OsCATC</i> showed the phenotype of LMMs and reduced starch metabolism. Taken together, the current study results revealed a novel role of <i>OsCATC</i> in starch metabolism in addition to validating previously studied functions of CATs. |
||
| Journal | Int J Mol Sci | |
| Country | China | |
| Volume | 23(7) | |
| Pages | ||
| Year | 2022 | |
| PubMed ID | 35409186 | |
| PubMed Central ID | 8998287 | |
| DOI | 10.3390/ijms23073827 | |
| URL | - | |
| Relation | ||
| Gene | AGPL1 AGPL3 NOE1 PR1B RSOSPR10 SSI SSIIB | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
|
Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
