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Reference Detail
| Reference | ||
|---|---|---|
| Author | Prakash S., Rai R., Zamzam M., Ahmad O., Peesapati R., Vijayraghavan U. | |
| Title | OsbZIP47 Is an Integrator for Meristem Regulators During Rice Plant Growth and Development. | |
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Abstract: Stem cell homeostasis by the WUSCHEL-CLAVATA (WUS-CLV) feedback loop is generally conserved across species; however, its links with other meristem regulators can be species-specific, rice being an example. We characterized the role of rice <i>OsbZIP47</i> in vegetative and reproductive development. The knockdown (KD) transgenics showed meristem size abnormality and defects in developmental progression. The size of the shoot apical meristem (SAM) in 25-day <i>OsbZIP47KD</i> plants was increased as compared to the wild-type (WT). Inflorescence of KD plants showed reduced rachis length, number of primary branches, and spikelets. Florets had defects in the second and third whorl organs and increased organ number. <i>OsbZIP47KD</i> SAM and panicles had abnormal expression for CLAVATA peptide-like signaling genes, such as <i>FON2-LIKE CLE PROTEIN1</i> (<i>FCP1</i>), <i>FLORAL ORGAN NUMBER 2</i> (<i>FON2</i>), and hormone pathway genes, such as cytokinin (CK) <i>ISOPENTEYLTRANSFERASE1</i> (<i>OsIPT1</i>), <i>ISOPENTEYLTRANSFERASE 8</i> (<i>OsIPT8</i>), auxin biosynthesis <i>OsYUCCA6, OsYUCCA</i>7 and gibberellic acid (<i>G</i>A) biosynthesis genes, such as <i>GRAIN NUMBER PER PANICLE1</i> (<i>GNP1/OsGA20OX1</i>) and <i>SHORTENED BASAL INTERNODE</i> (<i>SBI/OsGA2ox4</i>). The effects on <i>ABBERANT PANICLE ORGANIZATION1</i> (<i>APO1</i>), <i>OsMADS16</i>, and <i>DROOPING LEAF</i> (<i>DL</i>) relate to the second and third whorl floret phenotypes in <i>OsbZIP47KD</i>. Protein interaction assays showed OsbZIP47 partnerships with RICE HOMEOBOX1 (OSH1), RICE FLORICULA/LEAFY (RFL), and OsMADS1 transcription factors. The meta-analysis of KD panicle transcriptomes in <i>OsbZIP47KD</i>, <i>OsMADS1KD</i>, and <i>RFLKD</i> transgenics, combined with global OSH1 binding sites divulge potential targets coregulated by OsbZIP47, OsMADS1, OSH1, and RFL. Further, we demonstrate that OsbZIP47 redox status affects its DNA binding affinity to a <i>cis</i> element in <i>FCP1</i>, a target locus. Taken together, we provide insights on <i>OsbZIP47</i> roles in SAM development, inflorescence branching, and floret development. |
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| Journal | Front Plant Sci | |
| Country | India | |
| Volume | 13 | |
| Pages | 865928 | |
| Year | 2022 | |
| PubMed ID | 35498659 | |
| PubMed Central ID | 9044032 | |
| DOI | 10.3389/fpls.2022.865928 | |
| URL | - | |
| Relation | ||
| Gene | APO1 BZIP47 CLE402 DL FON2 GNP1 LHS1 OSH1 RFL SBI SPW1 YUCCA6 YUCCA7 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
