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Reference Detail
| Reference | ||
|---|---|---|
| Author | Yu N., Liang Y., Wang Q., Peng X., He Z., Hou X. | |
| Title | Transcriptomic analysis of OsRUS1 overexpression rice lines with rapid and dynamic leaf rolling morphology. | |
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Abstract: Moderate leaf rolling helps to form the ideotype of rice. In this study, six independent OsRUS1-GFP overexpression (OsRUS1-OX) transgenic rice lines with rapid and dynamic leaf rolling phenotype in response to sunlight were constructed. However, the mechanism is unknown. Here, RNA-Seq approach was utilized to identify differentially expressed genes between flag leaves of OsRUS1-OX and wildtype under sunlight. 2920 genes were differentially expressed between OsRUS1-OX and WT, of which 1660 upregulated and 1260 downregulated. Six of the 16 genes in GO: 0009415 (response to water stimulus) were significantly upregulated in OsRUS1-OX. The differentially expressed genes between WT and OsRUS1-OX were assigned to 110 KEGG pathways. 42 of the 222 genes in KEGG pathway dosa04075 (Plant hormone signal transduction) were differentially expressed between WT and OsRUS1-OX. The identified genes in GO:0009415 and KEGG pathway dosa04075 were good candidates to explain the leaf rolling phenotype of OsRUS1-OX. The expression patterns of the 15 genes identified by RNA-Seq were verified by qRT-PCR. Based on transcriptomic and qRT-PCR analysis, a mechanism for the leaf rolling phenotype of OsRUS1-OX was proposed. The differential expression profiles between WT and OsRUS1-OX established by this study provide important insights into the molecular mechanism behind the leaf rolling phenotype of OsRUS1-OX. |
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| Journal | Sci Rep | |
| Country | China | |
| Volume | 12(1) | |
| Pages | 6736 | |
| Year | 2022 | |
| PubMed ID | 35468979 | |
| PubMed Central ID | 9038715 | |
| DOI | 10.1038/s41598-022-10784-x | |
| URL | - | |
| Relation | ||
| Gene | CK1 DHN1 HKT1 IAA6 LEA3-1 LOX1 NHX1 PDR4 PIN5A PSAO PSBR RAB16A RAB16B RCN1 RR2 RUS1 _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
