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Reference Detail
| Reference | ||
|---|---|---|
| Author | Um T., Choi J., Park T., Chung P.J., Jung S.E., Shim J.S., Kim Y.S., Choi I.Y., Park S.C., Oh S.J., Seo J.S., Kim J.K. | |
| Title | Rice <i>microRNA171f/SCL6</i> module enhances drought tolerance by regulation of flavonoid biosynthesis genes. | |
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Abstract: Plants have evolved sophisticated defense systems to enhance drought tolerance. These include the microRNA (miRNA) group of small noncoding RNAs that act as post-transcriptional regulators; however, details of the mechanisms by which they confer drought tolerance are not well understood. Here, we show that <i>osa-MIR171f</i>, a member of <i>osa</i>-<i>MIR171</i> gene family, is mainly expressed in response to drought stress and regulates the transcript levels of <i>SCARECROW-LIKE6-I</i> (<i>SCL6-I</i>) and <i>SCL6-II</i> in rice (<i>Oryza sativa</i>). The <i>SCL6</i> genes are known to be involved in shoot branching and flag leaf morphology. <i>Osa-MIR171f-</i>overexpressing (<i>osa</i>-<i>MIR171f</i>-OE) transgenic plants showed reduced drought symptoms compared with non-transgenic (NT) control plants under both field drought and polyethylene glycol (PEG)-mediated dehydration stress conditions. Transcriptome analysis of <i>osa-MIR171f</i>-OE plants and <i>osa-mir171f</i>-knockout (K/O) lines generated by clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) revealed that <i>osa</i>-mature-miR171a-f (<i>osa</i>-MIR171) regulates the expression of flavonoid biosynthesis genes, consequently leading to drought tolerance. This upregulation in the <i>osa-MIR171f-</i>OE plants, which did not occur in NT control plants, was observed under both normal and drought conditions. Our findings indicate that <i>osa</i>-MIR171 plays a role in drought tolerance by regulating <i>SCL6-I</i> and <i>SCL6-II</i> transcript levels. |
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| Journal | Plant Direct | |
| Country | unknown | |
| Volume | 6(1) | |
| Pages | e374 | |
| Year | 2022 | |
| PubMed ID | 35028494 | |
| PubMed Central ID | 8743358 | |
| DOI | 10.1002/pld3.374 | |
| URL | - | |
| Relation | ||
| Gene | 4CL2 ARC3 CHI7 CHS CYP93G1 CYP93G2 F3'H F3'H8 GRAS31 HAM1 MIR171F TCM5 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
