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| Reference | ||
|---|---|---|
| Author | Wu Q., Liu Y., Huang J. | |
| Title | CRISPR-Cas9 Mediated Mutation in <i>OsPUB43</i> Improves Grain Length and Weight in Rice by Promoting Cell Proliferation in Spikelet Hull. | |
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Abstract: grain weight, a crucial trait that determines the grain yield in rice, is influenced by grain size. Although a series of regulators that control grain size.have been identified in rice, the mechanisms underlying grain development are not yet well understood. In this study, we identified OsPUB43, a U-box E3 ubiquitin ligase, as an important negative regulator determining the gain size and grain weight in rice. Phenotypes of large grain are observed in <i>OsPUB43</i> mutants, whereas overexpression of <i>OsPUB43</i> results in short grains. Scanning electron microscopy analysis reveals that <i>OsPUB43</i> modulates the grain size.mainly by inhibiting cell proliferation in the spikelet hull. The OsPUB43 protein is localized in the cytoplasm and nucleus. The <i>OsPUB43</i> mutants display high sensitivity to exogenous BR, while <i>OsPUB43</i>-OE lines are hyposensitive to BR. Furthermore, the transient transcriptional activity assay shows that OsBZR1 can activate the expression of <i>OsPUB43</i>. Collectively, our results indicate that OsPUB43 negatively controls the gain size by modulating the expression of BR-responsive genes as well as MADS-box genes that are required for lemma/palea specification, suggesting that OsPUB43 has a potential valuable application in the enlargement of grain size.in rice. |
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| Journal | Int J Mol Sci | |
| Country | China | |
| Volume | 23(4) | |
| Pages | ||
| Year | 2022 | |
| PubMed ID | 35216463 | |
| PubMed Central ID | 8877319 | |
| DOI | 10.3390/ijms23042347 | |
| URL | - | |
| Relation | ||
| Gene | BZR1 CYCB1;1 DLT FST KRP1 MFO1 PAP2 PUB43 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
