Update info.Tools - Reference - Detail
Reference Detail
| Reference | ||
|---|---|---|
| Author | Park J.R., Kim E.G., Jang Y.H., Jan R., Farooq M., Ubaidillah M., Kim K.M. | |
| Title | Applications of CRISPR/Cas9 as New Strategies for Short Breeding to Drought Gene in Rice. | |
|
Abstract: Recent unpredictable climate change is the main reason for the decline in rice yield. In particular, drought stress is a major constraint in reducing yield and quality for rice at rainfed agriculture areas, such as Asia and South America. CRISPR/Cas9 provides an effective solution for gene function study and molecular breeding due to specific editing of targeted genome sequences. In addition, CRISPR/Cas9 application can significantly reduce the time required to develop new cultivars with improved traits compared to conventional complex and time-consuming breeding. Here, drought-induced gene <i>Oryza sativa Senescence-associated protein</i> (<i>OsSAP</i>) was edited by CRISPR/Cas9. To investigate the possible role of <i>OsSAP</i> in drought stress, genome-editing plants were subjected to drought stress until the soil moisture content reached 20%, and the reactive oxygen species (ROS) scavenging efficiency of genome-editing plants were decreased. When the genome-editing plants were subjected to drought stress, survival rate, shoot length, root length, content of chlorophyll number of tiller, and 1,000-grain weight decreased, and more H2O2 and O2 - were detected in leaves. In addition, expression levels of several critical stress-related transcription factors were decreased in the <i>OsSAP</i> genome-editing plant. These results suggest that <i>OsSAP</i> function as a positive regulator during drought stress response in rice. We analyzed the expression of <i>OsSAP</i> and <i>Cas9</i> in T0 and T1 plants as well as T2 seeds. As the course of generation advancement progressed, <i>Cas9</i> expression remained stable or weakened but the <i>OsSAP</i> expression was continuously removed from the T0 plant. The coefficient of variation (CV) in both T1 plants and T2 seeds was lower than 5%. Overall, our results suggest that CRISPR/Cas9 could be a novel and important tool for efficiently generating specific and inheritable targeted genome editing in rice, with short breeding cycles. |
||
| Journal | Front Plant Sci | |
| Country | South Korea | |
| Volume | 13 | |
| Pages | 850441 | |
| Year | 2022 | |
| PubMed ID | 35283882 | |
| PubMed Central ID | 8908215 | |
| DOI | 10.3389/fpls.2022.850441 | |
| URL | - | |
| Relation | ||
| Gene | BZIP72 CATA DHN1 DREB2A LEA3-1 NAC5 NAC6 _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
|
Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
