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Reference Detail
| Reference | ||
|---|---|---|
| Author | Liu C., Li N., Lu Z., Sun Q., Pang X., Xiang X., Deng C., Xiong Z., Shu K., Yang F., Hu Z. | |
| Title | CG and CHG Methylation Contribute to the Transcriptional Control of OsPRR37-output Genes in Rice. | |
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Abstract: Plant circadian clock coordinates endogenous transcriptional rhythms with diurnal changes of environmental cues. OsPRR37, a negative component in the rice circadian clock, reportedly regulates transcriptome rhythms, and agronomically important traits. However, the underlying regulatory mechanisms of OsPRR37-output genes remain largely unknown. In this study, whole genome bisulfite sequencing and high-throughput RNA sequencing were applied to verify the role of DNA methylation in the transcriptional control of OsPRR37-output genes. We found that the overexpression of <i>OsPRR37</i> suppressed rice growth and altered cytosine methylations in CG and CHG sequence contexts in but not the CHH context (H represents A, T, or C). In total, 35 overlapping genes were identified, and 25 of them showed negative correlation between the methylation level and gene expression. The promoter of the hexokinase gene <i>OsHXK1</i> was hypomethylated at both CG and CHG sites, and the expression of <i>OsHXK1</i> was significantly increased. Meanwhile, the leaf starch content was consistently lower in <i>OsPRR37</i> overexpression lines than in the recipient parent Guangluai 4. Further analysis with published data of time-course transcriptomes revealed that most overlapping genes showed peak expression phases from dusk to dawn. The genes involved in DNA methylation, methylation maintenance, and DNA demethylation were found to be actively expressed around dusk. A DNA glycosylase, namely ROS1A/DNG702, was probably the upstream candidate that demethylated the promoter of <i>OsHXK1</i>. Taken together, our results revealed that CG and CHG methylation contribute to the transcriptional regulation of OsPRR37-output genes, and hypomethylation of <i>OsHXK1</i> leads to decreased starch content and reduced plant growth in rice. |
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| Journal | Front Plant Sci | |
| Country | China | |
| Volume | 13 | |
| Pages | 839457 | |
| Year | 2022 | |
| PubMed ID | 35242159 | |
| PubMed Central ID | 8885545 | |
| DOI | 10.3389/fpls.2022.839457 | |
| URL | - | |
| Relation | ||
| Gene | AGO18 AGO1A AGO1B AGO1D BBS1 DNG701 DNG704 HD2 HXK1 MADS18 NAS3 PT11 SDT TA2 _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
