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Reference Detail
| Reference | ||
|---|---|---|
| Author | Wu T., Zhang H., Bi Y., Yu Y., Liu H., Yang H., Yuan B., Ding X., Chu Z. | |
| Title | Tal2c Activates the Expression of <i>OsF3H04g</i> to Promote Infection as a Redundant TALE of Tal2b in <i>Xanthomonas oryzae</i> pv. <i>oryzicola</i>. | |
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Abstract: <i>Xanthomonas oryzae</i> delivers transcription activator-like effectors (TALEs) into plant cells to facilitate infection. Following economic principles, the redundant TALEs are rarely identified in Xanthomonas. Previously, we identified the Tal2b, which activates the expression of the rice 2-oxoglutarate-dependent dioxygenase gene <i>OsF3H03g</i> to promote infection in the highly virulent strain of <i>X. oryzae</i> pv. <i>oryzicola</i> HGA4. Here, we reveal that another clustered TALE, Tal2c, also functioned as a virulence factor to target rice <i>OsF3H04g</i>, a homologue of <i>OsF3H03g</i>. Transferring Tal2c into RS105 induced expression of <i>OsF3H04g</i> to coincide with increased susceptibility in rice. Overexpressing <i>OsF3H04g</i> caused higher susceptibility and less salicylic acid (SA) production compared to wild-type plants. Moreover, CRISPR-Cas9 system-mediated editing of the effector-binding element in the promoters of <i>OsF3H03g</i> or <i>OsF3H04g</i> was found to specifically enhance resistance to Tal2b- or Tal2c-transferring strains, but had no effect on resistance to either RS105 or HGA4. Furthermore, transcriptome analysis revealed that several reported SA-related and defense-related genes commonly altered expression in <i>OsF3H04g</i> overexpression line compared with those identified in <i>OsF3H03g</i> overexpression line. Overall, our results reveal a functional redundancy mechanism of pathogenic virulence in Xoc in which tandem Tal2b and Tal2c specifically target homologues of host genes to interfere with rice immunity by reducing SA. |
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| Journal | Int J Mol Sci | |
| Country | China | |
| Volume | 22(24) | |
| Pages | ||
| Year | 2021 | |
| PubMed ID | 34948428 | |
| PubMed Central ID | 8707247 | |
| DOI | 10.3390/ijms222413628 | |
| URL | - | |
| Relation | ||
| Gene | 2ODD2 2ODD3 BHLH6 CPS4 HSP18.0 TPS19 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
