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Reference Detail
| Reference | ||
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| Author | Zhou H., Zhou Y., Zhang F., Guan W., Su Y., Yuan X., Xie Y. | |
| Title | Persulfidation of nitrate reductase 2 Is Involved in l-Cysteine Desulfhydrase-Regulated Rice drought tolerance. | |
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Abstract: Hydrogen sulfide (H2S) is an important signaling molecule that regulates diverse cellular signaling pathways through persulfidation. Our previous study revealed that H2S is involved in the improvement of rice drought tolerance. However, the corresponding enzymatic sources of H2S and its regulatory mechanism in response to drought stress are not clear. Here, we cloned and characterized a putative <i>l-cysteine desulfhydrase</i> (<i>LCD</i>) gene in rice, which encodes a protein possessing H2S-producing activity and was named <i>OsLCD1</i>. Overexpression of <i>OsLCD1</i> results in enhanced H2S production, persulfidation of total soluble protein, and confers rice drought tolerance. Further, we found that nitrate reductase (NR) activity was decreased under drought stress, and the inhibition of NR activity was controlled by endogenous H2S production. Persulfidation of NIA2, an NR isoform responsible for the main NR activity, led to a decrease in total NR activity in rice. Furthermore, drought stress-triggered inhibition of NR activity and persulfidation of NIA2 was intensified in the <i>OsLCD1</i> overexpression line. Phenotypical and molecular analysis revealed that mutation of <i>NIA2</i> enhanced rice drought tolerance by activating the expression of genes encoding antioxidant enzymes and ABA-responsive genes. Taken together, our results showed the role of <i>OsLCD1</i> in modulating H2S production and provided insight into H2S-regulated persulfidation of NIA2 in the control of rice drought stress. |
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| Journal | Int J Mol Sci | |
| Country | China | |
| Volume | 22(22) | |
| Pages | ||
| Year | 2021 | |
| PubMed ID | 34829996 | |
| PubMed Central ID | 8624084 | |
| DOI | 10.3390/ijms222212119 | |
| URL | - | |
| Relation | ||
| Gene | APX1 BZIP23 CATA DREB1A LCD1 _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
