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Reference Detail
| Reference | ||
|---|---|---|
| Author | Liu X., Song L., Zhang H., Lin Y., Shen X., Guo J., Su M., Shi G., Wang Z., Lu G.D. | |
| Title | Rice ubiquitin-conjugating enzyme OsUBC26 is essential for immunity to the blast fungus Magnaporthe oryzae. | |
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Abstract: The functions of ubiquitin-conjugating enzymes (E2) in plant immunity are not well understood. In this study, OsUBC26, a rice ubiquitin-conjugating enzyme, was characterized in the defence against Magnaporthe oryzae. The expression of OsUBC26 was induced by M. oryzae inoculation and methyl jasmonate treatment. Both RNA interference lines and CRISPR/Cas9 null mutants of OsUBC26 reduced rice resistance to M. oryzae. WRKY45 was down-regulated in OsUBC26 null mutants. In vitro E2 activity assay indicated that OsUBC26 is an active ubiquitin-conjugating enzyme. Yeast two-hybrid assays using OsUBC26 as bait identified the RING-type E3 ligase UCIP2 as an interacting protein. Coimmunoprecipitation assays confirmed the interaction between OsUBC26 and UCIP2. The CRISPR/Cas9 mutants of UCIP2 also showed compromised resistance to M. oryzae. Yeast two-hybrid screening using UCIP2 as bait revealed that APIP6 is a binding partner of UCIP2. Moreover, OsUBC26 working with APIP6 ubiquitinateds AvrPiz-t, an avirulence effector of M. oryzae, and OsUBC26 null mutation impaired the proteasome degradation of AvrPiz-t in rice cells. In summary, OsUBC26 plays important roles in rice disease resistance by regulating WRKY45 expression and working with E3 ligases such as APIP6 to counteract the effector protein AvrPiz-t from M. oryzae. |
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| Journal | Mol. Plant Pathol. | |
| Country | China | |
| Volume | 22(12) | |
| Pages | 1613-1623 | |
| Year | 2021 | |
| PubMed ID | 34459564 | |
| PubMed Central ID | 8578843 | |
| DOI | 10.1111/mpp.13132 | |
| URL | - | |
| Relation | ||
| Gene | APIP6 C3H40 CK1A.3 CUL1 CZOGT3 DLN108 NAC40 PUB60 RFI2 RFP1 RLCK197 UBC26 UCIP11 UCIP12 UCIP13 UCIP15 UCIP16 UCIP4 UCIP5 UCIP9 WRKY45 ZFP1 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
