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Reference Detail
| Reference | ||
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| Author | Dangol S., Nguyen N.K., Singh R., Chen Y., Wang J., Lee H.G., Hwang B.K., Jwa N.S. | |
| Title | Mitogen-activated protein kinase OsMEK2 and OsMPK1 Signaling Is Required for Ferroptotic Cell Death in Rice-<i>Magnaporthe oryzae</i> Interactions. | |
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Abstract: Mitogen-activated protein kinase (MAPK) signaling is required for plant cell death responses to invading microbial pathogens. Iron- and reactive oxygen species (ROS)-dependent ferroptotic cell death occurs in rice (<i>Oryza sativa</i>) during an incompatible rice-<i>Magnaporthe oryzae</i> interaction. Here, we show that rice MAP kinase (OsMEK2 and OsMPK1) signaling cascades are involved in iron- and ROS-dependent ferroptotic cell death responses of rice to <i>M. oryzae</i> infection using <i>OsMEK2</i> knock-out mutant and <i>OsMEK2</i> and <i>OsMPK1</i> overexpression rice plants. The OsMPK1:GFP and OsWRKY90:GFP transcription factor were localized to the nuclei, suggesting that OsMPK1 in the cytoplasm moves into the nuclei to interact with the WRKY90. <i>M. oryzae</i> infection in Delta<i>OsMEK2</i> knock-out plants did not trigger iron and ROS accumulation and lipid peroxidation, and also downregulated <i>OsMPK1, OsWRKY90, OsRbohB</i>, and <i>OsPR-1b</i> expression. However, 35S:<i>OsMEK2</i> overexpression induced ROS- and iron-dependent cell death in rice. The downstream MAP kinase (<i>OsMPK1</i>) overexpression induced ROS- and iron-dependent ferroptotic cell death response to virulent <i>M. oryzae</i> infection. The small-molecule ferroptosis inhibitor ferrostatin-1 suppressed iron- and ROS-dependent ferroptotic cell death in 35S:<i>OsMPK1</i> overexpression plants. However, the small-molecule inducer erastin triggered iron- and lipid ROS-dependent, but <i>OsMEK2</i>-independent, ferroptotic cell death during <i>M. oryzae</i> infection. Disease (susceptibility)-related cell death was lipid ROS-dependent, but iron-independent in the Delta<i>OsMEK2</i> knock-out mutant during the late <i>M. oryzae</i> infection stage. These combined results suggest that <i>OsMEK2</i> and <i>OsMPK1</i> expression positively regulates iron- and ROS-dependent ferroptotic cell death, and blast disease (susceptibility)-related cell death was ROS-dependent but iron-independent in rice-<i>M. oryzae</i> interactions. |
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| Journal | Front Plant Sci | |
| Country | South Korea | |
| Volume | 12 | |
| Pages | 710794 | |
| Year | 2021 | |
| PubMed ID | 34408766 | |
| PubMed Central ID | 8365360 | |
| DOI | 10.3389/fpls.2021.710794 | |
| URL | - | |
| Relation | ||
| Gene | DSG1 MKK1 MPK6 NADP-ME2 PR1B RBOHB WRKY90 _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
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Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
