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Reference Detail
| Reference | ||
|---|---|---|
| Author | Park S.I., Park H.L., Bhoo S.H., Lee S.W., Cho M.H. | |
| Title | Biochemical and Molecular Characterization of the Rice Chalcone Isomerase Family. | |
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Abstract: Chalcone isomerase (CHI) is a key enzyme in flavonoid biosynthesis. In plants, CHIs occur in multigene families, and they are divided into four types, types I-IV. Type I and II CHIs are bona fide CHIs with CHI activity, and type III and IV CHIs are non-catalytic members with different functions. Rice contains seven CHI family genes (<i>OsCHI</i>s). Molecular analysis suggested that OsCHI3 is a type I CHI, and the other OsCHIs were classified into types III and IV. To elucidate their biochemical functions, OsCHI1, OsCHI3, OsCHI6, and OsCHI7 were expressed in <i>Escherichia coli</i>, and the recombinant OsCHI proteins were purified. An activity assay of recombinant OsCHIs showed that OsCHI3 catalyzed the isomerization of naringenin chalcone and isoliquiritigenin, whereas the other recombinant OsCHIs had no CHI activity. OsCHI3 also exhibited a strong preference to naringenin chalcone compared to isoliquiritigenin, which agrees well with the catalytic properties of type I CHIs. These results ascertain OsCHI3 to be a bona fide CHI in rice. <i>OsCHI3</i> and the other <i>OsCHI</i>s were expressed constitutively throughout the rice growth period and different tissues. <i>OsCHI3</i> expression was induced immediately in response to ultra-violet (UV) stress, suggesting its involvement in the biosynthesis of sakuranetin, a flavonoid phytoalexin in rice. |
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| Journal | Plants (Basel) | |
| Country | South Korea | |
| Volume | 10(10) | |
| Pages | ||
| Year | 2021 | |
| PubMed ID | 34685873 | |
| PubMed Central ID | 8540780 | |
| DOI | 10.3390/plants10102064 | |
| URL | - | |
| Relation | ||
| Gene | CHI1 CHI2 CHI3 CHI5 CHI6 CHI7 _ | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
|
Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
