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Reference Detail
| Reference | ||
|---|---|---|
| Author | He Y., Zhu M., Li Z., Jiang S., He Z., Xu S., Chen X., Hu Z., Zhang Z. | |
| Title | <i>IPA1</i> Negatively Regulates Early Rice Seedling Development by Interfering with Starch Metabolism via the GA and <i>WRKY</i> Pathways. | |
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Abstract: <i>Ideal Plant Architecture 1 (IPA1)</i> encodes SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 14 (SPL14) with a pleiotropic effect on regulating rice development and biotic stress responses. To investigate the role of <i>IPA1</i> in early seedling development, we developed a pair of <i>IPA1</i>/<i>ipal</i>-NILs and found that seed germination and early seedling growth were retarded in the <i>IPA1</i>-NIL. Analysis of the soluble sugar content, activity of amylase, and expression of the <i>alpha</i>-amylase genes revealed that the starch metabolism was weakened in the <i>IPA1</i>-NIL germinating seeds. Additionally, the content of bioactive gibberellin (GA) was significantly lower than that in the <i>IPA1</i>-NIL seeds at 48 h of imbibition. Meanwhile, the expression of GA synthesis-related gene <i>OsGA20ox1</i> was downregulated, whereas the expression of GA inactivation-related genes was upregulated in <i>IPA1</i>-NIL seeds. In addition, the expression of <i>OsWRKY51</i> and <i>OsWRKY71</i> was significantly upregulated in <i>IPA1</i>-NIL seeds. Using transient dual-luciferase and yeast one-hybrid assays, IPA1 was found to directly activate the expression of <i>OsWRKY51</i> and <i>OsWRKY71</i>, which would interfere with the binding affinity of GA-induced transcription factor OsGAMYB to inhibit the expression of <i>alpha</i>-amylase genes. In summary, our results suggest that <i>IPA1</i> negatively regulates seed germination and early seedling growth by interfering with starch metabolism via the GA and <i>WRKY</i> pathways. |
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| Journal | Int J Mol Sci | |
| Country | China | |
| Volume | 22(12) | |
| Pages | ||
| Year | 2021 | |
| PubMed ID | 34203082 | |
| PubMed Central ID | 8234402 | |
| DOI | 10.3390/ijms22126605 | |
| URL | - | |
| Relation | ||
| Gene | AMY1A AMY1C AMY3B AMY3C AMY3D AMY3E EPE1 GA2OX3 GA2OX6 GA2OX8 GAMYB GNP1 SUT1 WFP WRKY51 WRKY71 | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
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Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
