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| 著者 | Wang Z., Wang Q., Wei L., Shi Y., Li T., Hu K., Liu S., Zhong H., Liao J., Li Y., Zhang H., Huang Y. | |
| タイトル | UDP-<i>N</i>-Acetylglucosamine Pyrophosphorylase 2 (UAP2) and 1 (UAP1) Perform Synergetic Functions for Leaf Survival in Rice. | |
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Abstract: Functional inactivation of UDP-<i>N</i>-acetylglucosamine pyrophosphorylase 1 (UAP1) induces defense response-related lesion-mimic spots and subsequent early senescence in every newly grown leaf of the rice mutant <i>UAP1</i> after a short period's normal growth. However, the molecular mechanism of these leaves sustaining the short period's survival is still unknown. Phenotypic and molecular studies show that defense response-related lesion-mimic spots and early leaf senescence appear on the normally grown <i>UAP1</i> leaf and aggravate with the growth time. Bioinformatic analysis reveals that UAP proteins are evolutionarily conserved among eukaryotes, and there exists UAP2 protein except UAP1 protein in many higher organisms, including rice. Rice UAP2 and UAP1 proteins present high sequence identities and very similar predicted 3D structures. Transcriptional expression profile of the <i>UAP2</i> gene decreases with the appearance and aggravating of leaf spots and early senescence of <i>UAP1</i>, implying the role of the <i>UAP2</i> gene in maintaining the initial normal growth of <i>UAP1</i> leaves. Enzymatic experiments verified that the UAP2 protein performs highly similar UAP enzymatic activity with the UAP1 protein, catalyzing the biosynthesis of UDP-GlcNAc. And these two UAP proteins are found to have the same subcellular localization in the cytoplasm, where they most presumably perform their functions. Overexpression of the <i>UAP2</i> gene in <i>UAP1</i> plants succeeds to rescue their leaf mutant phenotype to normal, providing direct evidence for the similar function of the <i>UAP2</i> gene as the <i>UAP1</i> gene. The <i>UAP2</i> gene is mainly expressed in the young leaf stage for functions, while the <i>UAP1</i> gene is highly expressed during the whole leaf developmental stages. Based on these findings, it is suggested that <i>UAP2</i> and <i>UAP1</i> play key roles in rice leaf survival during its development in a synergetic manner, protecting the leaf from early senescence. |
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| 掲載誌 | Front Plant Sci | |
| Country | China | |
| 刊数 | 12 | |
| ページ | 685102 | |
| 発刊年 | 2021 | |
| PubMed ID | 34249055 | |
| PubMed Central ID | 8264299 | |
| DOI | 10.3389/fpls.2021.685102 | |
| URL | - | |
| 関連情報 | ||
| 遺伝子 | AMTR1 GABA-T ICL NAC58 PR10A PR1A SALT SPL29 UAP2 | |
| INSD | - | |
| 系統 |
野生イネ コアコレクション |
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| 突然変異系統(遺伝研 Collection) | - | |
| 種子不稔系統 | - | |
| 致死変異体系統 | - | |
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組織別発生段階 - 突然変異系統(遺伝子) |
- | |
| 栽培イネ品種(遺伝研 Collection) | - | |
| 組織別発生段階 | - | |
