Update info.Tools - Reference - Detail
Reference Detail
| Reference | ||
|---|---|---|
| Author | Xin W., Wang J., Li J., Zhao H., Liu H., Zheng H., Yang L., Wang C., Yang F., Chen J., Zou D. | |
| Title | Candidate Gene Analysis for Nitrogen Absorption and Utilization in Japonica Rice at the Seedling Stage Based on a Genome-Wide Association Study. | |
|
Abstract: Over-application of nitrogen (N) fertilizer in fields has had a negative impact on both environment and human health. Domesticated rice varieties with high N use efficiency (NUE) reduce fertilizer requirements, enabling sustainable agriculture. Genome-wide association study (GWAS) analysis of N absorption and utilization traits under low and high N conditions was performed to obtain 12 quantitative trait loci (QTLs) based on genotypic data including 151,202 single-nucleotide polymorphisms (SNPs) developed by re-sequencing 267 japonica rice varieties. Eighteen candidate genes were obtained by integrating GWAS and transcriptome analyses; among them, the functions of <i>OsNRT2.4</i>, <i>OsAMT1.2</i>, and <i>OsAlaAT</i> genes in N transport and assimilation have been identified, and OsJAZ12 and OsJAZ13 also play important roles in rice adaptation to abiotic stresses. A NUE-related candidate gene, <i>OsNAC68</i>, was identified by quantitative real-time PCR (qRT-PCR) analyses. <i>OsNAC68</i> encodes a NAC transcription factor and has been shown to be a positive regulator of the drought stress response in rice. Overexpression of <i>OsNAC68</i> significantly increased rice NUE and grain yield under deficient N conditions, but the difference was not significant under sufficient N conditions. NUE and grain yield significantly decreased under both N supply conditions in the <i>osbnac68</i> mutant. This study provides crucial insights into the genetic basis of N absorption and utilization in rice, and a NUE-related gene, <i>OsNAC68</i>, was cloned to provide important resources for rice breeding with high NUE and grain yield. |
||
| Journal | Front Plant Sci | |
| Country | China | |
| Volume | 12 | |
| Pages | 670861 | |
| Year | 2021 | |
| PubMed ID | 34149769 | |
| PubMed Central ID | 8212024 | |
| DOI | 10.3389/fpls.2021.670861 | |
| URL | - | |
| Relation | ||
| Gene | AMT1;2 FLO12 NAC14 NRT2.4 TIFY11D TIFY11E | |
| INSD | - | |
| Strain | Wild Core Collection | - |
| Induced Mutation Lines(NIG Collection) | - | |
| Sterile Seed Strain | - | |
|
Lethal Embryo Mutantion Strain |
- | |
|
Stages in Each Organ - Muant Lines (Gene) |
- | |
| Cultivated Varieties(NIG Collection) | - | |
| Stages in Each Organ | - | |
